功能化微流控细胞芯片的构建及初步应用

Construction and application of a functional microfluidic cell chip

目的构建一个结构简单,并有利于细胞贴壁培养的微流控芯片系统。方法利用生物交联剂将RGD肽共价连接在芯片微通道表面,通过与细胞膜表面整合素家族的特异性识别,促进细胞的黏附与贴壁生长;考察不同浓度RGD的功能化效果。结果化学交联法能将RGD有效的固定在芯片微通道表面,形成均匀一致的RGD层。RGD功能化处理的细胞芯片具有良好的生物相容性,可在无CO2的室温条件下实现较长时间的细胞培养。结论表面修饰能实现RGD肽在微流控芯片材料表面的固定并保持其生物活性,有利于细胞在微通道中的贴壁培养。

Objective To develop a simple microfluidic cell chip,which is enable in adherent cell culture.Methods Arginine-glycine-aspartic acid（RGD） peptide was covalently linked to the microchannel surface of cell chip with biological cross-linker.Through specific identification between RGD and membrane integrin,cells could be adhere to microchannel surface.The functionalization under different concentration of RGD were evaluated.Results RGD peptide was attached on the microchannel surface effectively and uniformly through chemical crosslinking.A549 cells were survival up to 12 h in the microchannel at room temperature without CO2 because of the good biocompatibility of functional chip.Conclusion Surface modification enables the RGD peptide link to microchannel surface and maintains its activity,and benefits to the cells adherent culture.