双向电泳在疾病研究中的应用

The Application Progresses of the Two Dimensional Electrophoresis in Biomedical Research

生命的表现形式最终是由蛋白质决定的。因此,对蛋白质组的研究具有很现实的意义。双向电泳(2-DE)技术作为蛋白质组学研究的一项核心技术,主要用于细胞、组织以及其他样本蛋白质提取物的分析。近年来,2-DE结合质谱(MS)技术被广泛用于差异蛋白的鉴定、疾病标志物的筛选、药物靶标的确定等,目前已经成为蛋白质组学研究的支撑技术,以其高通量、高分辨率和重复性被广泛应用到各个领域,特别是生物医学的研究中。本文就近年2-DE技术的应用进展,尤其在生物医学方面的贡献做一综述。

Research about proteomies is of great significance. Two-dimensional electrophoresis （2-DE） is a core technology of proteomics research, which is used for analysis of the protein extracted from cell, tissue and other samples. In recent years, 2-DE combined with mass spectrum （MS） technology is widely used to identify differential protein, to screen tumor markers, to detect drug targets and so on. Proteomies research has become key technology, with its high throughput, high resolution and repeatability, and is widely used in various fields, particularly in biomedical research. We provided here a short review about the application development of 2-DE, especially its contribution on biological medicine.