电针后血清对肿瘤坏死因子α诱导软骨细胞凋亡的影响

Effects of serum on tumor necrosis factor alpha induced chondrocyte apoptosis following electro-acupuncture

背景:电针具有活血化瘀、行气止痛、舒筋通络的作用,能有效缓解骨性关节炎的临床症状。目的:观察电针后血清对肿瘤坏死因子α诱导软骨细胞凋亡的影响。方法:采用机械-酶消化法分离3周龄SD大鼠膝关节软骨细胞,用10μg/L的肿瘤坏死因子α诱导体外培养软骨细胞的凋亡,同时给予正常大鼠血清、骨性关节炎大鼠血清、透明质酸钠治疗的骨性关节炎大鼠血清、及电针内、外膝眼5,15,30min治疗的骨性关节炎大鼠血清进行干预。结果与结论:电针内、外膝眼15,30min治疗的骨性关节炎大鼠血清干预24h,软骨细胞活力显著增高,凋亡显著减少。说明电针后血清能有效抑制肿瘤坏死因子α诱导的软骨细胞凋亡。

BACKGROUND：Electro-acupuncture（EA） can promote blood circulation,remove blood stasis,regulate vital energy circulation to relieve pain,relieve rigidity of muscles and activate collaterals,and effectively relieve symptoms of osteoarthritis.OBJECTIVE：To observe the effect of serum on tumor necrosis factor α（TNF-α） induced chontroctye apoptosis following EA.METHODS：Knee joint chondrocytes from 3-week-old SD rats were isolated using enzymatic digestion and apoptosis induced with 10 μg/L TNF-α in vitro.The normal group rats were routinely raised and did not receive any treatment.The osteoarthritis rats were routinely raised,intraarticularly injected with sodium hyaluronate（0.4 μL/g,once a week,for successive 5 weeks） ,or given EA at the Neixiyan and Waixiyan（5,15 and 30 min/d） ,and all rats were interfered with serum,and then the apoptotic rate of the chondrocytes was determined.RESULTS AND CONCLUSION：After 15 and 30 minutes of EA at the Neixiyan and Waixiyan,osteoarthritis rats were interfered with serum for 24 hours,the activity of chondrocytes was significantly increased and the apoptotic rate of the chondrocytes was significantly decreased.These findings suggest that serum can inhibit TNF-α induced chondrocyte apoptosis following EA.