坐骨神经损伤模型大鼠神经传导速度及损伤运动神经元内生长相关蛋白43表达与磁刺激干预

Effects of magnetic stimulation on nerve conduction velocity and the expression of growth associated protein 43 in rats after sciatic nerve injury

背景:磁刺激可促进损伤神经的修复。目的:观察磁刺激对大鼠损伤坐骨神经神经传导速度及相应水平脊髓运动神经元内生长相关蛋白43表达的影响。方法:将60只SD大鼠随机分为实验组(n=24)、模型组(n=24)和假手术组(n=12),用一新的长17cm的止血钳钳夹坐骨神经至第二扣,以21.95×103Pa维持10s制备损伤模型。造模后24h,实验组每天给予0.09T的磁刺激。结果与结论:造模后第2,4,8,12周,免疫组织化学染色显示实验组脊髓L4～5运动神经元生长相关蛋白43的表达较模型组相应时间点明显增高(P<0.05);造模后12周,电生理检测发现,与模型组比较,实验组再生神经传导速度加快,波幅升高,潜伏期缩短(P<0.05)。说明磁刺激能提高损伤坐骨神经的传导速度,增加其对应脊髓节段运动神经元中生长相关蛋白43的表达,对大鼠损伤坐骨神经的修复起促进作用。

BACKGROUND：Magnetic stimulation may promote the repair of nerve injury.OBJECTIVE：To explore the effect of magnetic stimulation on nerve conduction velocity and growth associated protein 43 expression of corresponding spinal motor neuron in rats after sciatic nerve injury.METHODS：A number of 60 SD rats were randomly assigned into 3 groups：experiment group（n=24） ,model group（n=24） and sham-operation group（n=12） .The mouse sciatic nerve was clamped by a new hemostatic forcep with a length of 17 centimeters.The clamping tension was adjusted by locking the 2nd interlocking teeth with a pressure of 21.95×103 Pa,lasting for 10 seconds to construct sciatic nerve injury model.At 24 hours after the model construction,the experiment mice were treated with a daily magnetic stimulation of 0.09 T.RESULTS AND CONCLUSION：According to immunohistochemical staining,at the 2nd,4th,8th and 12th weeks after model construction,the expression of growth associated protein 43 in L4-5 motor neurons in the experiment group was significantly higher than that of the model group at the same time point（P〈0.05） .Electrophysiology examination showed that compare with the model group,the regenerated nerve of the experiment group had faster conduction velocity,higher amplitude and shorter latent period at the 12th week after model construction（P〈0.05） .These results demonstrate that the magnetic stimulation can speed up the nerve conduction of injured sciatic nerve,increase the expression of growth associated protein 43 in corresponding motor neurons and promote the repair of sciatic nerve injury in rats.