葛根素联合雌二醇对去卵巢大鼠骨质疏松症的治疗作用(英文)

Puerarin combined with estradiol for treatment of osteoporosis in ovariectomized rats

背景:雌激素治疗绝经后骨质疏松症产生的不良反应较大。目的:观察小剂量葛根素联合雌二醇对去卵巢大鼠骨组织的影响。方法:120只健康雌性大白鼠等分成只切除卵巢旁的一小块脂肪垫的假手术组和切除双侧卵巢的去卵巢模型组、葛根素组、雌二醇组及葛根素+雌二醇组。1周后葛根素组、雌二醇组和雌二醇+葛根素组分别皮下注射葛根素(50mg/kg,1次/d)、雌二醇(200μg/kg,2次/周)和雌二醇(100μg/kg,2次/周)+葛根素(25mg/kg,1次/d)。结果与结论:去卵巢模型组大鼠骨组织稀疏,骨钙、磷水平和骨密度明显低于假手术组(P<0.01),而经雌二醇和/或葛根素治疗后大鼠骨组织形态明显改善、骨钙、磷水平和骨密度明显升高。其中小剂量的葛根素联合雌二醇治疗与较大剂量的葛根素或雌二醇治疗效果接近。提示较小剂量的雌二醇与葛根素联合治疗也可对去卵巢大鼠骨质疏松症产生良好的治疗效果。

BACKGROUND：Estradiol produces great adverse effects in treatment of postmenopausal osteoporosis.OBJECTIVE：To investigate the effects of small dose of estradiol combined with estradiol on bone tissue of ovariectomized rats.METHODS：A total of 120 healthy female rats were randomly and evenly divided into five groups：sham-operated（only resection of a small block of para-ovarian fat pad） ,ovariectomized（without drugs） ,puerarin,estradiol,and puerarin ＋ estradiol.One week later,rats from the puerarin,estradiol and puerarin ＋ estradiol groups were subcutaneously injected with puerarin（50 mg/kg,once a day） ,estradiol（200 μg/kg,twice a week） ,estradiol puerarin（25 mg/kg,once a day） ＋（100 μg/kg,twice a week） ,respectively.RESULTS AND CONCLUSION：In the ovariectomized group,rat bone tissue was sparse,bone calcium and phosphorus levels as well as bone mineral density were significantly lower compared with sham-operated group（P〈0.01） .After estradiol and/or puerain treatment,rat bone tissue morphology was obviously improved,and bone calcium and phosphorus levels and bone mineral density were significantly increased.The above-mentioned indices were similar after small dose of puerarin combined with estradiol treatment and high dose of puerarin combined with estradiol treatment.These findings suggest that small dose of estradiol combined with estradiol treatment yields satisfactory curative effects in treatment of osteoporosis in ovariectomized rats.