补肾健脾方干预去势大鼠骨骼肌caspase-3和caspase-8的表达

Effects of the prescription for tonifying kidney and strengthening spleen on caspase-3 and caspase-8 expression in skeletal muscle of ovariectomized rats

背景:骨骼肌与骨质疏松症关系密切,导致其衰退的主要原因是细胞的凋亡,这种凋亡可能是Caspase家族蛋白所介导的。目的:探讨补肾健脾方对去势大鼠骨骼肌caspase-3和caspase-8调控作用。方法:SD大鼠48只等随机分为对照组、模型组、中药组、西药组,后3组大鼠摘除双侧卵巢建立骨质疏松模型,对照组仅切除周围少量脂肪组织。造模2周后,中药组给予补肾健脾方(2.979g/kg)灌胃,西药组给予戊酸雌二醇片(0.104mg/kg)灌胃,模型组和对照组给予蒸馏水灌胃,1次/d。结果与结论:①干预12周后,双能X线骨密度仪测量发现,相比于对照组,模型组大鼠左侧股骨的骨密度值和骨矿含量均明显降低(P<0.01);而中药组和西药组的骨密度值均高于模型组(P<0.05),且2组骨密度值和骨矿含量接近(P>0.05)。②酶标免疫分析显示,与对照组相比,模型组骨骼肌中Caspase-3和Caspase-8表达水平明显上升(P<0.05)。中药能显著降低大鼠骨骼肌中Caspase-3的表达水平(P<0.05),而西药能显著减低Caspase-8表达水平(P<0.05)。③说明补肾健脾方对卵巢切除所致的骨质疏松症有明显的治疗作用,能明显提高骨密度,而且能显著减低Caspase-3的水平,但不能显著减低Caspase-8水平,显示补肾健脾方不是通过死亡受体途径去抑制细胞凋亡的。

BACKGROUND：Studies have shown that there is a close relationship between skeletal muscle and osteoporosis.Apoptosis is a main reason of skeletal muscle aging,which may be mediated by the proteins of caspase family.OBJECTIVE：To study the mechanism of the prescription for tonifying kidney and strengthening spleen on preventing and treating osteoporosis and observe the regulation of the prescription on caspase-3 and caspase-8 expression in skeletal muscle of ovariectomized rats.METHODS：Forty-eight female non-pregnant SD rats（6 months） were randomly and evenly divided into four groups：control,ovariectomized,estrogen and traditional Chinese herb.After establishing model,the rats of traditional Chinese herb group were intragastrically administered with the prescription for tonifying kidney and strengthening spleen（2.979 g/kg） .The rats of estrogen group received an intragastric administration of estradiol valerate（0.104 mg/kg） .The rats of the other groups were intragastrically given distilled water,once a day.RESULTS AND CONCLUSION：Dual energy X-ray absorptiometry results showed that compared with control group,bone mineral density（BMD） and bone mineral content（BMC） in rat left femur were significantly lower in the model group（P 0.01） .The BMD was significantly greater in the estrogen and traditional Chinese herb groups than in the model group（P 0.05） and the BMD and BMC were similar between the estrogen and traditional Chinese herb groups（P 0.05） .Enzyme linked immunosorbent assay results showed that compared with the control group,caspase-3 and caspase-8 expression in the skeletal muscle was significantly increased in the model group（P 0.05）;caspase-3 expression was significantly decreased in the traditional Chinese herb group（P 0.05） ,and caspase-8 expression was significantly decreased in the estrogen group（P 0.05） .These findings suggest that the prescription for tonifying kidney and strengthening spleen exhibits obvious therapeutic effects on ovariectomy-induced osteoporosis,can increase bone mineral density and obviously decrease caspase-3 expression,but it can not markedly decrease caspase-8 expression.This indicates that the prescription does not inhibit apoptosis by death receptor signal pathway.