改良酶学方法获得异种血管脱细胞支架

In vitro construction of decellularized vascular xenograft by improved enzymatic method

背景:扩大脱细胞基质的孔径与孔隙率,保证其作为移植物的力学特性,是目前血管组织工程研究的热点之一。目的:观察改良的酶学方法处理异种血管的力学性能和组织相容性。方法:取猪的颈动脉作为基质,采用传统胰蛋白酶-EDTA加1%TritonX-100和0.1%氨水顺序脱细胞方案,并做了适当的方法改进,延长胰酶处理的时间,分别为胰酶处理4h,5h,6h。结果与结论:经组织学分析,脱细胞基质中无细胞成分,脱细胞的支架结构完整,随着胰酶处理时间的延长,其基质内弹力板破坏明显,孔径和孔隙率逐渐增大,缝合强度与爆破强度相对于自然血管虽略有降低,但差异无显著性意义。组织成分胶原蛋白含量也有所下降,尤其胰酶延长至6h组,其胶原蛋白含量明显低于正常未处理的自然血管(P<0.05)。结果显示延长胰蛋白酶处理时间后得到的脱细胞血管基质,具有良好的孔隙率、生物力学性能和组织相容性。

BACKGROUND： How to expand the pore size and porosity of the acellular matrix to facilitate the post-cell re-cultivation requirement, and simultaneously the graft must ensure its mechanical requirements, is one of the hotspots in vascular tissue engineering. OBJECTIVE： To observe the mechanical properties and histocompatibility of decellularized vascular xenograft vascular grafts constructed by the improved enzymatic method. METHODS： Porcine carotid arteries were decellularized using conventional trypsin-EDTA plus 1% TritonX-100 and 0.1% ammonia in order for 4, 5, and 6 hours. RESULTS AND CONCLUSION： Porcine carotid arteries were decellularized by trypsin, ammonium hydroxide and non-ionic detergent to extract all the cell components of the grafts successfully. To obtain better pore size and porosity of the acellular scaffold, we changed the methods by extending the time of trypsin. By extending the time of trypsin methods, the better pore size and porosity of the acellular scaffold was obtained. Biomechanics, porosity characteristics of scaffold can meet the requirements of tissue engineered vessel.