慢性粒细胞白血病肿瘤干细胞的免疫功能

Immuno-modulatory effect of cancer stem cells from chronic myeloid leukemia patients

背景:研究认为间充质干细胞可能是骨髓造血微环境的免疫保护位点。由于慢性粒细胞白血病存在造血微环境异常和免疫异常,所以推测间充质干细胞可能在慢性粒细胞白血病的病理过程中扮演了一个重要角色。目的:观察慢性粒细胞白血病骨髓来源的肿瘤干细胞的免疫学特征,比较其与正常人来源的间充质干细胞是否存在免疫功能的异常。方法:分离正常人和慢性粒细胞白血病患者的骨髓间充质干细胞,分别检测它们对T细胞周期、活化、抑制和增殖的作用。结果与结论:慢性粒细胞白血病和正常志愿者骨髓来源的间充质干细胞形态和表型没有差异,慢性粒细胞白血病患者来源的间充质干细胞抑制T细胞增殖的作用减弱,抑制T细胞周期及活化的能力减弱,慢性粒细胞白血病患者抑制T细胞凋亡的作用增强。提示慢性粒细胞白血病患者骨髓来源的间充质干细胞存在明显的免疫调节功能缺陷,如果使用慢性粒细胞白血病患者自体的间充质干细胞移植治疗可能不是一种很好的选择,对于骨髓增生异常综合征患者最好是选用异基因的间充质干细胞移植。

BACKGROUND：Studies have suggested that mesenchymal stem cells may be immune protection sites consisting of the bone marrow micro-environment.As chronic myeloid leukemia hematopoietic microenvironment abnormalities and immune abnormalities exist,mesenchymal stem cells in chronic myelogenous leukemia presumably play an important role in the pathological process.OBJECTIVE：To observe the immunological characteristics and the existence of immune dysfunction of bone marrow-derived cancer stem cells from chronic myeloid leukemia in comparison with normal mesenchymal stem cells.METHODS：Bone marrow mesenchymal stem cells were separated from the normal subjects and patients with chronic myeloid leukemia to detect the effect on T cell cycle,activation,inhibition and proliferation.RESULTS AND CONCLUSION：There were no differences between mesenchymal stem cell from chronic myeloid leukemia patients and normal volunteers in cell morphology and phenotype,but the ability of mesenchymal stem cells from chronic myelogenous leukemia patients was decreased in inhibiting T cell proliferation,T cell cycle and activation and increased in T cell apoptosis.All these indicate that mesenchymal stem cells from chronic myelogenous leukemia patients have obvious immune function defects,which are not a preferred choice for autologous mesenchymal stem cell transplantation.Allogeneic mesenchymal stem cell transplantation is the best method to be used in patients with myelodysplastic syndrome.