当归枸杞制剂诱导脐血单个核细胞分化为肝细胞后人白蛋白的表达

Angelica sinensis(oliv.) diels and lycium chinense mill preparations induce the differentiation of cord blood mononuclear cells into hepatocyts:Expression of human albumen

背景:部分中药能够促进造血干细胞的生长及分化。目的:观察当归、枸杞制剂促进人脐血单个核细胞生长分化为肝细胞的作用、作用条件及对肝衰竭的治疗效果。方法:分离人脐血单个核细胞,加入不同剂量的当归及枸杞制剂,比较不同剂量当归及枸杞制剂对脐血单个核细胞及其转化为肝细胞后人白蛋白mRNA及人甲胎蛋白mRNA表达的影响。腹腔内注射D-氨基半乳糖建立大鼠肝衰竭模型,随机分为生理盐水对照组、脐血单个核细胞组、脐血单个核细胞与当归、枸杞制剂灌胃和腹腔内注射免疫抑制剂不同组合8组,检测大鼠肝功能变化。结果与结论:90mg/L当归、40mg/L枸杞制剂可促进单个核细胞的增殖和分化,并加快脐血单个核细胞分化为肝细胞,两者联合无药物协同作用。单独应用当归与枸杞对大鼠肝损伤均有一定的修复作用,与单个核细胞联合均可促进单个核细胞向肝细胞分化,增强修复肝损伤的作用,并且当归的修复效果优于枸杞。加用环磷酰胺后并不能增强当归或枸杞联合单个核细胞的治疗效果。

BACKGROUND：Some traditional Chinese medicine can promote the differentiation and growth of human hematopoietic stem cells.OBJECTIVE：To observe the promotion effect of angelica sinensis（oliv.） diels and lycium chinense mill preparation for the differentiation of human umbilical cord blood stem cells（HUCBSCs） into hepatocytes in vitro and in vivo.METHODS：HUCBSCs were separated and cultured in different doses of angelica sinensis（oliv.） diels and lycium chinense mill preparation to screen the suitable dosage for HUCBSCs growth.Albumen mRNA and alpha fetoprotein mRNA expression were detected by RT-PCR.The liver failure models of rats were established after D-aminogalactose was injected intraperitoneally.The survival rats with acute hepatic injury were randomly divided into eight groups to detect the changes of liver function.RESULTS AND CONCLUSION：90 mg/L angelica sinensis（oliv.） diels and 40 mg/L lycium chinense mill preparation was suitable for HUCBSCs proliferation and differentiation.Both angelica sinensis（oliv.） diels and lycium chinense mill preparation could promote and speed up the differentiation of HUCBSCs into hepatocytes,and the former one was superior to the latter one.However,there was no synergistic effect.The difference between groups treated with/without cyclophosphamide was not obvious.