骨髓间充质干细胞静脉移植治疗大鼠脊髓损伤

Intravenous transplantation of bone marrow mesenchymal stem cells in the treatment of spinal cord injury in rats

背景:干细胞移植可以重建中枢神经系统的结构和功能,近年来引起了广泛的关注。目的:尾静脉移植骨髓间充质干细胞观察其对大鼠脊髓损伤修复的影响并探讨其机制。方法:密度梯度离心法结合贴壁筛选法分离骨髓间充质干细胞。将成年雌性Wistar大鼠以动脉瘤夹钳夹法制备大鼠脊髓损伤模型后随机分为细胞移植组和对照组,分别干预。结果与结论:细胞移植组大鼠BBB后肢功能评分恢复优于对照组(P<0.05)。自损伤后第30天开始,细胞移植组大鼠运动诱发电位潜伏期及体感诱发电位P1波潜伏期的恢复均优于对照组(P<0.05),并持续至实验结束。细胞移植组大鼠脊髓内在损伤中心及头、尾端距离脊髓损伤中心1cm处均可见阳性细胞。说明尾静脉注射移植骨髓间充质干细胞可以促进脊髓损伤大鼠神经功能恢复,其机制可能与移植细胞迁移到损伤部位,分化为神经元样和神经胶质细胞样细胞,并分泌或促进宿主分泌神经营养因子有关。

BACKGROUND：Transplantation of stem cells can rebuild the structure and function of injured central nervous system,and has attracted wide attention in recent years.OBJECTIVE：To explore the effects and mechanisms of bone marrow mesenchymal stem cells（BMSCs） transplanted through the vena caudalis on neurological recovery of rats with spinal cord injury.METHODS：BMSCs were separated with density gradient centrifugation and adherent screening methods.Forty adult female Wistar rats were used to establish the models of spinal cord injury using aneurysm clips,and then randomized into two groups：control group and BMSCs transplantation group.RESULTS AND CONCLUSION：The BMSCs transplantation group showed higher BBB scores than the control group（P 0.05）.Since the 30th day after injury,the motor evoked potential latency and somatosensory evoked potential P1 wave latency of the BMSCs transplantation group were shorter than those of the control group（P 0.05）,and these phenomena continued until the end of experiment.Positive cells could be noted at the sites 1 cm distant from the injured centre of the spinal cord.BMSCs transplantation via the vena caudalis can remarkably promote the recovery of neural function in rats with spinal cord injury,and the mechanism may be related to that transplanted BMSCs can survive,migrate and differentiate into neuron like cells and neuroglia like cells,and can promote the expression of neurotrophic factors.