人脐带间充质干细胞诱导成骨及治疗骨缺损

Human umbilical cord mesenchymal stem cells induce ossification and treat bone defects

背景:人脐带间充质干细胞在成骨及组织器官修复方面具有更强的扩增能力及低免疫原性,其成集落生长潜能及成骨时间早于骨髓等其他来源间充质干细胞。目的:观察脐带间充质干细胞诱导成骨及移植治疗骨缺损的临床效果。方法:应用组织块贴壁法提取人脐带间充质干细胞,体外行成骨诱导并通过光镜观察、茜素红染色、碱性磷酸酶染色、Ⅰ型胶原的表达等证实其体外成骨能力;对临床骨缺损病例行人脐带间充质干细胞移植,移植后定期复查骨缺损部骨痂生长状况。结果与结论:体外诱导证实人脐带间充质干细胞具有明确的成骨作用。骨缺损患者在人脐带源间充质干细胞移植后2个月X射线见左股骨髁上骨折部位骨块间隙模糊,骨折外周形成明显的骨痂,骨折断端相连,断端骨折线依然存在;移植后3个月见骨痂间已经形成明显骨性连接。证实脐带间充质干细胞具有体外诱导成骨及体内移植修复骨缺损作用。

BACKGROUND：Umbilical cord mesenchymal stem cells have greater expansion capacity and low immunogenicity in ossification and tissue and organ repair,and their growth potential into a colony is earlier than mesenchymal stem cells of the bone marrow and other sources.OBJECTIVE：To observe the clinical effect of umbilical cords mesenchymal stem cells on inducing ossification and treating bone defects.METHODS：Human umbilical cord mesenchymal stem cells were isolated by the tissue block culture.In vitro osteogenic ability was confirmed by the morphology changes of human umbilical cord mesenchymal stem cells,alizarin red staining,alkaline phosphatase staining,and type Ⅰ collagen expression.Human umbilical cord mesenchymal stem cells transplantation was performed in patients with bone defects,and conditions of callus growth were regularly reviewed after transplantation.RESULTS AND CONCLUSION：In vitro induction experiments confirmed that umbilical cord mesenchymal stem cells had a clear osteogenic role.Two months after cell transplantation,X-rays showed bony callus formation,fracture interspace vague.Osseous connection formation was found at 3 months after cell transplantation.It was confirmed that umbilical cord mesenchymal stem cells exerted a role of osteogenic induction,and could repair bone defects via in vivo cell transplantation.