乙肝病毒外膜大蛋白检测及在病毒复制诊断中的临床应用

The detection and clinical application of HBV large protein(HBV-LP) in diagnosing viral replication

目的探讨乙肝外膜大蛋白(HBV-LP)检测用于乙肝患者的临床诊断意义。方法采用酶联免疫吸附实验(ELISA)检测HBV-LP、电化学发光发检测病毒血清标志物,荧光定量聚合酶链式反应(PCR)方法对患者HBV DNA进行检测。结果乙肝病毒血清标志物相同模式患者血清中HBV-LP与HBV DNA检出率无显著差异(P>0.05);110例小三阳乙肝患者血清中HBV-LP HBeAg阳性检出率与HBV DNA阳性率差异无统计学意义,二者的检出一致率为78.18%;HBV-LP吸光度(OD值)与HBV DNA呈正相关关系(r=0.986)。结论在HBeAg阴性的乙肝患者中HBV-LP与HBV DNA有较高检出一致率,与HBV DNA有良好的正相关关系,HBV-LP可反映乙肝病毒复制水平。

Objective To explore the clinical significance of HBV large protein（HBV-LP） in diagnosing viral replication,we detected the HBV-LP,HBV DNA and the hepatitis B viral markers（HBV M） in the serum of the patients infected with HBV.Methods HBV-LP and HBV M were analyzed by using ELISA.HBV DNA was quantitatively detected using real-time quantitative PCR.Results 1.No significant difference between the detectable rate of HBV DNA and HBV-LP was found in the same HBV M（P0.05）.2.No significant difference between the positive rate of HBV DNA and HBV-LP was found in HBeAg negative patients.There was significant difference between the detectable rate of HBV DNA and HBV preS1 was found in HBeAg negative patients.3.The contents of serum HBV-LP was positively correlated with HBV DNA copies（r=0.986）.Conclusion There is a perfect correlation between the positive rate of HBV-LP and HBV DNA,and HBV-LP is a reliable serological marker that can reflect the replication of HBV.