固相萃取用于血浆中抗癫痫药物的提取

Extraction of the antiepilepsy drugs in plasma by SPE

目的：探讨固相萃取法用于血浆中抗癫痫药物的提取。方法：以活化的ＯＤＳ 柱（４０μｍ） 为固相萃取柱，二氯甲烷为洗脱剂，洗脱液浓缩后以ＨＰＬＣ 法测定药物的浓度。结果：苯巴比妥、苯妥英在５ ．００ ～６０ ．００μｇｍｌ 范围内，卡马西平在２ ．５０ ～２０ ．００μｇｍｌ 范围内线性关系良好，ｒ 分别为０ ．９９８０ ，０ ．９９９９ ，０ ．９９９０ ；绝对回收率分别为５９ ．６０ ％～６８ ．７ ％ ，５８ ．９ ％ ～６８ ．０ ％ ，５３ ．８ ％ ～５７ ．３ ％ ；相对回收率分别为９２ ．２ ％ ～９８ ．６ ％ ，９７ ．１ ％ ～１０２ ．６ ％ ，９７ ．９ ％ ～１０２ ．０ ％ ，ＲＳＤ＜ ６ ％ ；血浓监测结果与经传统的液液萃取后所得结果非常接近。结论：固相萃取法操作简单、省时、提取干净，可用于血浆中抗癫阅药物的提取。

OBJECTIVE:To extract the antiepilepsy drugs in plasma by solid phase extraction(SPE).METHOD:Actived ODS columns(40μm) were used as SPE columns and CH 2CL 2 was used as solvent to extract the drugs in plasma,their concentractions were determined by HPLC.RESULTS:For phenobarbital phenytoin and carbamazepine,the linear range were 5.00～60.00μg/ml(r=0.9980),5.00～60.00μg/ml(r=0.9999),and 2.50～20.00μg/ml(r=0.9990) respectively,the absolute rewveries were 59.60%～68.7%,53.80%～68.0^ and 55.4%～57.3% respectively,and the relative rewveries were 92.2%～98.6%,97.1%～102.6% and 97.9%～102.0% respectively(RSD<6%).Concentractions of drugs in plasma determined by SPE were similar to those by liquid phase extraction.CONCLUSION:SPE is rapid,simple,clean and suitable to the extraction of antiepilepsy drugs in plasma.