摘要
利用发根农杆菌ATCC15834诱导获得乌桕毛状根,研究了不同培养条件对乌桕毛状根生长及次生代谢产物累积的影响。结果表明:乌桕毛状根生产量随培养时间的增加而增加,在第6周时达到最大产量(0.6668g/瓶,DW);1～4周内暗培养更利于毛状根的生长,其后则光照培养更好;在相同培养时间内,毛状根在B5和1/2MS基本培养基上产量大于1/4MS、1/8MS及WP培养基;HPLC测定结果表明,1/4MS较其它培养基更利于多酚类代谢产物的累积。
Hairy roots were induced from Sapium sebiferum by using Agrobacterium rhizogenes ATCC15834.Influence of cultural conditions on the growth of hairy roots and its second metabolites accumulation were studied.The results showed that the yield of hairy roots increased with the cultural time and reached the maximum（0.668 g/flask,dry weight）at the sixth week;the yield of hairy roots in B5 and 1/2MS was more than in 1/4MS,1/8MS or WP basal medium;HPLC detection results showed that the polyphenols accumulation in 1/4MS was better those in other medium.
出处
《北方园艺》
CAS
北大核心
2011年第22期103-105,共3页
Northern Horticulture
关键词
乌桕
毛状根
培养
次生代谢产物
累积
Sapium sebiferum
hairy roots
culture
second metabolites
accumulation
