摘要
以陕北地区野生的山丹丹鳞片为试材,采用单因子和正交设计研究了不同浓度IBA和NAA及其组合对山丹丹组培快繁的影响。结果表明:适合山丹丹初代培养的培养基为MS+6-BA 1.5mg/L+NAA 0.01mg/L,芽诱导率达到100%;继代培养为MS+6-BA 2.5mg/L+IBA0.2mg/L,繁殖系数达到9.8;生根培养为1/2MS+IBA 0.3mg/L,生根率达到100%。
Taking Shaanxi wild Plant Lilium pumilum DC.pellet flake material,using single factor and orthogonal design,to study the effect of different concentration of IAA,NAA on the tissue culture and rapid propagation of Lilium pumilum DC.process.The results showed that MS＋6-BA 1.5 mg/L＋NAA 0.01 mg/L suitable for primary culture,the bud induction rate was 100%.MS＋6-BA 2.5 mg/L＋IBA 0.2 mg/L was for subculture,propagation coefficient was 9.8.Rooting was 1/2MS＋IBA 0.3 mg/L,rooting rate of 100%.
出处
《北方园艺》
CAS
北大核心
2011年第22期115-117,共3页
Northern Horticulture
基金
国家大学生文化素质基地资助项目(YDWH10-16)
陕西省自然科学基金资助项目(2009JM3001-3)
延安市科技计划资助项目(2009kn-26)
延安大学科技创新资助项目(D2010-158)
关键词
野生
山丹丹
快繁
wild
Lilium pumilum DC.
rapid propagation
