离子液体微波辅助提取葛根总黄酮

Microwave-assisted extraction of flavonoids in Radix Puerariae with ionic liquids solution

采用微波辅助提取技术,以葛根总黄酮提取率为指标,以溴化1-丁基-3-甲基咪唑([bmim]Br)水溶液为提取剂提取葛根总黄酮.分别考察了提取时间、提取温度、微波功率、提取液浓度以及固液比对葛根总黄酮提取率的影响.结果显示,葛根总黄酮提取最佳条件为:提取剂[bmim]Br水溶液浓度为1.0mol/L,最佳固液比为120,微波功率为500W,提取温度为70℃,萃取时间8min,其粗黄酮的提取率为8.05%.与传统的乙醇提取方法相比,萃取率要高13.5%,使用的溶剂量少,微波功率低,快速高效,且提取率高.

A Microwave-assisted extraction（MAE） method of flavonoids from radix puerariae byBr solution were investigated.The extraction condition of solid-liquid ratio,concentration of solvent,time,temperature and microwave power were researched,respectively.The MAE conditions were that the solvent was 1.0 mol/LBr solution,the solid/liquid（g：mL） ratio was 120,the microwave power was 500 W,the extraction temperature was 70 ℃,and time was 8 min.Under these MAE conditions,the yield of flavonoids in Radix Puerariae is 8.05%.Compared with traditional ethanol extraction method,the yield of the extraction byBr solution increased by 13.5%.The advantage of the method is Low-solvent consumption,low microwave power,fast,efficient and high-yield.