慢性丙型肝炎患者血清抗组织谷氨酰胺转移酶抗体的检测及临床研究

Monitoring and clinical research of IgA anti-tissue transglutaminase antibodies in patients with chronic hepatitis C

目的了解抗组织谷氨酰胺转移酶(tTG)抗体在慢性丙型肝炎患者中的表达。方法选取56例慢性丙型肝炎患者,采用ELISA法检测血清Ig-A型抗tTG抗体,采用间接免疫荧光法测定血清自身抗体ANA、AMA和SMA,采用BECKMAN全自动生化分析仪测定ALT和球蛋白。结果 23例肝硬化患者中IgA-抗tTG抗体阳性9例,与IgA-抗tTG抗体阴性肝硬化患者相比,ALT、自身抗体、免疫球蛋白均无明显差异。结论丙肝后肝硬化患者体内存在较高的IgA-抗tTG抗体表达水平,可能与肝硬化引起的门脉高压有关。

【Objective】 To investigate the expression of IgA anti-tissue transglutaminase（tTG） antibodies in patients with chronic hepatitis C.【Methods】 Fifty-six patients with chronic hepatitis C were evaluated for IgA anti-tTG antibodies by ELISA.Serum level of ANA,AMA and SMA were detected in all the patients using indirect immunofluorescence assay.Levels of ALT and globulins were also measured.【Results】 Anti-tTG antibodies of Nine patients in twenty-three cirrhosis cases were positive.There were no significant difference in ALT,auto-antibodies and globulins between anti-tTG antibodies positive group and anti-tTG antibodies negative group.【Conclusion】 There is a high prevalence of anti-tTG antibodies in patients with chronic hepatitis C,and it is associated with portal hypertension.