绞股蓝总皂甙对内毒素休克家兔血压及一氧化氮的影响

Effect of Gypenosides on Mean Arterial Pressure and Nitric Oxide Levels in Rabbits with Endotosin Induced Shock

目的 探讨绞股蓝总皂甙(GPS) 对休克家兔血压及一氧化氮的影响。方法 动物静脉麻醉后,随机分为3 组,即对照组、内毒素组、内毒素+ 绞股蓝总皂甙组。取血测定NO 水平并测量血压。给动物注射内毒素引起家兔休克为动物模型,动物处死后,取血再次测定NO 水平。结果 对照组实验前后NO 没有出现明显的变化( P0 .05) 。内毒素(LPS) 组平均动脉血压(MAP) 在实验后4h、6h 时明显低于实验前和对照组( P< 0 .01) ,实验后NO 明显高于实验前。GSP组实验后6h 时,MAP低于实验前和对照组( P< 0 .05) ,但高于LPS组( P< 0 .05) ,实验后的NO 浓度高于实验前和对照组( P< 0.05) ,但低于LPS组实验后的NO浓度( P< 0 .05) 。结论 绞股蓝总皂甙对休克家兔血压有保护作用,对NO

Aim To investigate the effects of Gypenosides on mean arterial pressure and nitric oxide levels in rabbits with endotoxin indueced shock. Methods Rabbits were randomly divided into three groups, i.e. control, endotoxin (LPS), and endotoxin plus Gypenosides (GPS). The animals were anaethetized with intravenous injection of 20% ethyl urethane. Mean arterial pressare was determined. Nitric oxide levels were determined by the method of colorimetric analysis. Results All the investigated values in control group were not significantly changed during the experiment. The plasma level of nitric oxide in GPS group was lower than that in LPS group but higher than that in control group after administration of Gypenosides. MAP showed significant difference between LPS and GPS groups, and both of them were lower than control group. The investigation demonstrated that Gepenosides prominently improved the mean arterial pressure of the rabbits, and delayed the animal death caused by shock. Conclusion It appeared to the author that the mechanism of the improvement might involve decrease of NO level and the improvement of shock.