摘要
目的是分析大鼠分泌LH的GnRH受体后信号转导机制。将GTH细胞内PKC兴奋或抑制后,用高频GnRH脉冲刺激,再用ELISA法检测其LH分泌量,并与空白组比较。结果表明,细胞PKC活性显著影响LH的分泌。得出结论,PKC-Ca2+是GnRH脉冲刺激引起的LH分泌的GnRH受体后信号转导途径之一。
The aim was to analyze the GnRH post-receptor signal transduction mechanism of the secretion of LH in the mammalian.The GTH cells was stimulated by GnRH at high frequencies after PKC in them was excited or inhibited.LH was detected by ELISA,which was compared with the blank comparison group.The PKC level in GTH cells influenced the expression of LH significantly.PKC-Ca2＋ was one of the GnRH post-receptor signal transduction pathways that the cells stimulated by GnRH impulse secret LH.
出处
《中国农学通报》
CSCD
北大核心
2011年第29期42-45,共4页
Chinese Agricultural Science Bulletin
基金
国家自然科学基金"不同脉冲频率GnRH调节大鼠GTH表达的信号转导机制研究"(30571356)
青岛农业大学高层次人才启动基金"ICA作用GTH细胞的受体后信号转导机制研究"(630810)
实验技术课题"小鼠茜素红
Alcian蓝重染色骨组织标本制作方法的改进"(SYJK09-05)
教学研究课题"<中兽医学>教学方法的研究"(XJG0906)
广州中医药大学博士后科研启动金"不同脉冲频率GnRH调节大鼠GTH表达的信号转导机制研究"(B3YH1008)
