部分地区传染性胃肠炎病毒株S基因的序列分析

Sequence Analysis of S Gene of Transmissible Gastroenteritis Virus Isolated from Different Regions

猪传染性胃肠炎(Porcine Transmissible Gastraenteritis,TGE)是猪的一种高度接触性肠道疾病。收集不同地区猪传染性胃肠炎病毒株的S基因为靶序列,利用生物信息学方法分析不同地区TGEV病毒株S基因的同源性和进化性关系,序列分析包括遗传距离测定、进化树分析和碱基替换分析等。结果表明S基因在TGEV不同毒株间高度保守,但S基因存在腺嘌呤碱基(A)向胸腺嘧啶(T)突变的倾向。通过对TGEVS基因核苷酸序列分析可为深入研究TGEV的毒力变化和流行病学提供参考。

Transmissible gastroenteritis （TGE） is an acute highly contagious disease of pigs caused by transmissible gastroenteritis virus （TGEV）.S gene sequences of several TGEV strains derived from different regions were analyzed for the homology and evolution.Bioinformatics methods for the analysis of the S gene,including genetic distance measurement,phylogenetic tree analysis,and residue substitution analysis,showed that the S gene was highly conservative between each TGEV strains,but there were the tendency of adenine nucleotide （A） mutation to thymidine nucleotide （T）.This results shows that the nucleotide sequence analysis can form a base or further study on the virulence and epidemiological study of TGEV infections.