不同花型牡丹品种亲缘关系的SRAP分析

SRAP Analysis of Genetic Relationships of the Different Tree Peony Flower Forms

为了从分子水平上揭示牡丹品种间的遗传差异,用SRAP-PCR技术对20个牡丹品种进行基因组DNA多态性分析,并用UPGMA法构建系统发育树。结果表明,20对引物扩增出131条清晰、稳定的DNA条带,其中90条谱带表现多态性,多态性检测率为69.6%,表明受试牡丹品种之间含较丰富的遗传多态性。UPGMA聚类结果表明,部分花型相同的品种相聚,但部分花型不同的品种亦相聚。说明聚类结果与牡丹品种的花型间可能有一定的相关性,但并未有完全一致的关系。

In order to reveal the genetic differences among tree peony cultivars,the genomic polymorphisms of 20 tree peony cultivars were studied by SRAP-PCR technique and the phylogenetic tree was constructed with UPGMA method.The results showed that 131 clear and stable DNA bands were amplified by 20 pairs SRAP primers,among which 90 bands were polymorphic,the percentage of polymorphic bands was 68.7%.It suggested there were abundant genetic diversity among tree peony cultivars.The UPGMA results indicated that the partial cultivars with the same or different flower forms sometimes clustered together in different case.These results demonstrated that the cluster results were correlated with flower forms of tree peony cultivars,but it had not similar relationships.