^(60)Co-γ射线对芹菜种子的辐射效应

Effect of ^(60)Co-γ Ray Irradiation on Celery Seed

以3个芹菜品种的干种子为材料,用不同剂量的60Co-γ射线进行辐照处理,研究辐照对种子活力和M1代苗期生长及生理指标的影响。结果表明,辐射对3个品种的效应一致。当辐照剂量达到或高于200Gy时,芹菜种子的萌发能力和种子活力显著下降,当辐照剂量为300Gy时,种子没有发芽能力。60Co-γ射线辐照影响了芹菜M1代幼苗的生长和生理代谢,当辐照剂量达到或大于200Gy时,幼苗的株高下降了50%～60%;50、100、150Gy的辐照剂量刺激了幼苗的超氧化物歧化酶(SOD)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)和谷光甘肽还原酶(GR)活性的增加,50、100、150Gy的辐照剂量下超氧阴离子(O2.-)产生速率和丙二醛(MDA)的含量和对照差异不显著,当辐照剂量达到或超过200Gy时,幼苗SOD、POD和APX活性显著下降,且O2.-产生速率和MDA含量显著上升,说明低于或等于150Gy的辐照不会对芹菜幼苗的生长造成影响。综合分析所测定的指标,芹菜干种子的适宜诱变剂量为100～150Gy。

The dry seeds of celery were irradiated by different doses of 60Coγ ray,the effects of radiation on seed vigor and M 1 seedlings physiological characteristics were investigated.The results showed the effect of 60Coγ ray irradiation on three cultivars seeds were similar.When the doses of 60Coγ ≥ 200 Gy,the germination and vigor of seed decreased remarkably.And there was no germination after irradiated by 300 Gy 60Coγ.There was a negative effect of radiation on M 1 seedlings.The M 1 seedlings height declined with 50%-60% when the doses of 60Coγ ≥ 200 Gy.When the doses were 50,100,150 Gy,the SOD,POD,APX,GR activity increased compare with 0 Gy,and there was not remarkable difference on O 2 ·production rate and MDA content between 50,100,150 Gy 60Co-γ and 0 Gy 60Co-γ.However,when the doses of 60Co-γ ≥200 Gy,the SOD,POD,APX activity decreased remarkably and O 2 · production rate,MDA content increased remarkably.The 60Coγ ≤ 150 Gy did not inhibit the growth of celery seedlings.Therefore,it was concluded that the optimum doses of celery dry seeds was 100-150 Gy.