草莓抗白粉病生理生化指标研究

Study on Index of Physiological and Biochemical in Resistance to Strawberry Powdery Mildew

为研究不同草莓品种对白粉病的抗性,选取5种对白粉病抗性不同的草莓品种为试材,测定草莓叶片中叶绿素和可溶性糖含量以及苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)和超氧化物歧化酶(SOD)活性变化。结果表明,草莓叶片的叶绿素含量及POD、PAL、PPO活性随品种白粉病抗性的增强而增加,SOD活性随品种抗白粉病能力增强而降低;可溶性糖含量随品种白粉病抗性的增强无明显变化规律。进行方差分析得到不同品种供试材料的叶绿素含量、PPO活性、PAL活性差异极显著,可以用叶绿素含量、PPO活性、PAL活性这些生理生化指标来衡量不同草莓品种对白粉病抗性强弱。

It was studied the resistance to powdery mildow（Sphaerotheca fuliginea） of 5 different strawberry cultivars.The content of soluble carbohytrate and changes in activity of specific enzymes such as PAL,POD,PPO and SOD were detected.The results showed that the content of chloroplast of the leaves and the activity of PAL,POD,PPO were increased as the resistance strenghened,the activity of SOD was reduced as the resistance strenghened,while the change in content of soluble carbohytrate was negligible.The result of variance analysis illustrated the significant difference in the content of chloroplast and the activity of PAL and PPO between different cultivars,which confirmed that the content of chloroplast and the activity of PAL,PPO could used as indicator for resistance to powdery mildow（Sphaerotheca fuliginea） of strawberry.