b-DNA技术在诺如病毒检测中的应用

Application of b-DNA technique in detection of norovirus

目的评价b-DNA技术在诺如病毒检测中的应用价值。方法收集医院2009年6-10月肠道门诊就诊腹泻患者204例,分别采用RT-PCR和b-DNA法进行检测,比较两种方法的检出率。结果在所检测的204例腹泻患者标本中,RT-PCR法检出26例,阳性率为12.7%,b-DNA法检出33例,阳性率为16.2%,两种方法的检出率差异无统计学意义。结论由于b-DNA技术无需抽提纯化RNA、反转录和PCR扩增,操作步骤简单,所需实验设备条件少,具有广阔地临床应用前景。

OBJECTIVE To evaluate the efficiency of branched DNA（b-DNA） in the detection of norovirus from feces samples. METHODS The faces samples of 204 patients with diarrhea were collected and tested by RT-PCR and b-DNA respectively. The detection efficiency of the two methods were compared. RESULTS Out of the samples, the positive rate of RT-PCR method was 12. 7% and of b-DNA method was 16. 2%. There was no significant difference between the two methods. CONCLUSION b-DNA method shows great prospect in norovirus detection in clinic for low cost and sample procedure without RNA extraction, reverse transcription and PCR amplification.