人肝癌细胞移植瘤QGY-9204中细胞调亡和坏死并存现象的初步分析

Observations on the Concurrence of Apoptosis and Necrosis in Human Transplanted Hepatoma Tumor QGY-9204

关于细胞调亡和坏死的判别标准及其相互关系已逐渐成为研讨的热点．研究表明，p53基因的表达导致细胞周亡，抑制肿瘤形成．在将Ad／p53导人人肝癌细胞移植瘤QGY－9204的研究中发现，实验组与对照组的移植瘤中均出现凋亡特征指标（DNA梯型条带、凋亡小体等）．而离体培养的肝癌细胞QGY－7703并未检出细胞凋亡现象．那么，实体瘤的DNA梯型条带与调亡和坏死有何关系？研究表明，瘤体内细胞阔亡和坏死并存，与对照组相比，实验组的瘤体积较小，坏死面积小，凋亡比例较高．对移植瘤QGY－9204中细胞调亡和坏死的特征及其并存现象的可能原因进行了初步分析，推断：在该移植瘤内，凋亡和坏死并非是细胞死亡的两种相互排斥的选择，它们可能相继发生和互为影响．

It was observed in previous studies that expression of p53 gene could trigger cell apoptosis in vitro and inhibit tumor formation following the transfection of the human hepatoma cell line QGY-7703 with p53 cDNA.Unexpectedly, generally accepted characteristics of apoptosis, were observed in both QGY-77O3 cell-derived transplantable hepatoma QGY-92O4 injected with Ad/p53 and the control (injected with medium). Furthermore, both apoptotic and necrotic cells were detected in the Ad/p53 injected tumors and the control. However,the incidence of apoptosis in the treated tumors was higher than that in the control. These phenomena suggest that the process of apoptosis and necrosis may not be exclusive.