姜黄素对人肺成纤维细胞凋亡及细胞周期的影响

Effect of curcumin on the apoptosis and the cell cycle of human lung fibroblasts

目的研究姜黄素对人肺成纤维细胞(human lung fibroblasts,HLFs)体外生长、细胞周期及凋亡的影响。方法应用CCK-8、Annexin-V/PI、PI流式细胞分析术、免疫印迹法,检测姜黄素对细胞的增殖、凋亡、细胞周期和Bax蛋白表达的影响。结果姜黄素对HLFs的增殖有抑制作用,可促进HLFs的早期凋亡,对细胞周期可产生G2/M期阻滞,增加HLFs的Bax蛋白的表达。结论姜黄素可抑制HLFs的增殖并能诱导其凋亡,其机制可能与细胞的G2/M期阻滞及凋亡相关蛋白的表达增强有关。

Objective To study the effects of curcumin on the growth,cell cycle and apoptosis of human lung fibroblasts（HLFs）in vitro.Methods The proliferation of HLFs was detected by CCK-8 assay,the apoptosis was detected by Annexin-V/PI fluorescence staining,cell cycle was analyzed by flow cytometry,Bax protein was detected by Western blot method.Results The results showed that curcumin could inhibit the growth of HLFs and promote the early apoptosis of HLFs,it also could arrest the cell cycle in G2/M phase and upregulate the expression of Bax protein in HLFs.Conclusion Curcumin may inhibit the proliferation of HLFs and induce its apoptosis in vitro;its mechanism probably related to the arrest of cell cycle in G2/M phase and enhanced expression of Bax protein.