结扎阻塞性黄疸大鼠肝动脉对肝细胞凋亡影响

Influence on liver cell apoptosis by blocking the hepatic artery in rats with obstructive jaundice

目的通过比较阻塞性黄疸大鼠行胆肠内引流时,切断肝固有动脉与不切断肝固有动脉动物模型的血清胆红素、肝脏病理形态学改变,以及肝细胞凋亡率改变,初步探讨阻塞性黄疸大鼠行胆肠内引流时结扎或切断肝固有动脉的安全性和可行性及其机制。方法 48只雄性SD大鼠,随机分为A、B、C、D 4组。结扎大鼠胆总管,建立阻塞性黄疸模型,7 d后行胆肠内引流。A、B组不结扎切断肝固有动脉,C、D组结扎切断肝固有动脉。A、C组胆肠内引流后7 d处死,B、D组胆肠内引流后14 d处死。收集血液和肝脏组织送检测。结果 A组大鼠死亡1只,死亡时间为胆肠内引流后第3 d,病死率8.33%;D组死亡2只,为胆肠内引流后第10、12 d,病死率16.67%。病死率差异无统计学意义(P>0.05)。血清胆红素(TB IL)、凋亡率(AI)C组高于A组,D组高于B组,差异有统计学意义(P<0.05)。病理形态学改变(如肝脏组织纤维化、肝细胞坏死及炎细胞浸润)肝固有动脉结扎组均重于非结扎组。结论阻塞性黄疸大鼠行胆肠内引流时结扎切断肝固有动脉不安全可行。阻塞性黄疸大鼠行胆肠内引流时结扎切断肝固有动脉对肝细胞凋亡率有较大影响。

Objective This objective used the animal models of obstructive jaundice rats with biliary drainage compared serum bilirubin,pathological changes,apoptosis between two groups of which blocked the hepatic artery or not after 7 days and 14 days respectively,invesgated whether the cutting of hepatic artery is safe and feasible in obstructive jaundice rats with biliary enteric drainage.Methods 48 male SD rats were randomly divided into A,B,C,D groups,and made into obstructive jaundice by ligated common bile duct,adopted enteric drainage and cutted C,D group hepatic artery 7 days later.A,C scarificed 7 days later and B、D groups 14 days later,collected speciments to test.Results Serum bilirubin and apoptosis rate of rats ligated hepatic artery were significantly higher than not ligated（P〈0.05）,liver fibrosis,necrosis and inflammatory cell infiltration became serious.Conclusion It is cautious to ligate hepartic artery of rats with obstructive jaundice when biliary enteric drainaged.2.It is effect on liver cell apoptosis rat with cutting the hepatic artery in rats with ob structive jaundice.