池州地区大肠埃希菌和肺炎克雷伯菌产超广谱β-内酰胺酶的基因型分布

Genotype distribution of extended-spectrum β-lactamases in ESBLs-producing escherichia coli and klebsiella pneumoniac in Chizhou

目的研究池州地区分离的大肠埃希菌(E.coli)和肺炎克雷伯菌(K.pn)质粒编码的产超广谱β-内酰胺酶(ES-BLs)基因分布情况。方法收集大肠埃希菌152株,肺炎克雷伯菌36株,采用美国临床实验室标准委员会(NCCLS)规定的ESBLs表型筛选和确证试验,确定本地区ESBLs的发生率,并通过特异性引物、聚合酶链反应(PCR)反应、及核酸电泳分析上述菌株所携带的耐药基因并进行基因分型。结果本地区17.1%的大肠埃希菌27.7%肺炎克雷伯菌产ESBLs,其中大肠埃希菌耐药质粒编码TEM型,SHV型和非TEM,SHV型基因的百分率分别为:65.4%,26.9%,7.7%,肺炎克雷伯菌分别为20%,40%,40%。结论本地区临床分离产ESBLs大肠埃希菌和肺炎克雷伯菌耐药情况严重,且其耐药表型也不尽相同。临床上严格控制和合理使用抗生素已经刻不容缓。

Objective To study the genotype distribution of ESBLs about Escherichia coli and Klebsiella pneumoniac in Chizhou.Methods To collect 152 Escherichia coli and 36 Klebsiella pneumoniac and using the method of National Committee for Clinical Laboratory Standards to filt and ascertainment the Escherichia coli and Klebsiella pneumoniac with ESBLs.Extraction the plasmid of the bacterium with ESBLs,and amplification by polymerase chain reaction were applied.Nucleate electrophoretic analysis genotyping of ESBLs.Results The percent of Escherichia coli ESBLs positive is 17.1%,Klebsiella pneumoniac is 27.7%.Among the Escherichia coli typing TEM is 65.4% typing SHV is 26.9% remnan is 7.7%.Among the Klebsiella pneumoniac typing TEM is 20% typing SHV is 40% remnan is 40%.Conclusion ESBLs-producing E.coli and K.pneumoniae strains isolated from Chizhou are highly resistant.Their resistance phenotypes were different.The use of antibiotic must be controlled and rational use antibiotic at clinical.