西菲律宾海比科尔陆架深海沉积物古菌多样性研究

The diversity of archaea in deep-sea sediments from the West Philippine Sea Bicol shelf

通过构建16S rRNA基因文库,对西太平洋西菲律宾海东板比科尔陆架5个不同层位沉积物样品中的古菌的多样性进行了研究,并获得了465个有效克隆63个OTUs(Operational Taxonomic Units)。通过16S rRNA序列与GenBank已知序列的同源性比较及构建系统进化树的结果显示,古菌序列分别来自泉古菌(Crenarchaeota)和广古菌(Euryarchaeota),以Marine Benthic Group B(MBGB)、Marine Group I(MGI)、Miscellaneous Crenarchaeotic Group(MCG)和Marine Benthic Group D(MBGD)为主。少量序列为Marine Hydrothermal Vent Group(MHVG)。研究结果表明,该垂直分布的5个不同层位的沉积物样品仅有5个古菌类群,古菌群落多样性并不高,且5个层位中的优势古菌类群稍有差异。研究结果揭示了比科尔陆架深海古菌垂向分布特征,为今后大范围自然海区古菌生态学研究提供科学参考。

The archaeal diversity and phylogenesis of the sediments samples from the Bicol shelf of the West Philippine Sea for the West Pacific Ocean were analyzed by the 16S rRNA gene clone library method. Totally 465 cloning sequences were obtained and divided into 63 OTUs. Phylogenetic analysis showed that the archaeal sequences were from Crenarchaeota and Euryarchaeota. The majority of the archaeal phylotypes were Marine Benthic Group B （MBGB）, Marine Group I （MGI）, Miscellaneous Crenarchaeotic Group （MCG）, and Marine Benthic Group D. A few sequences fell into Marine Hydrothermal Vent Group （MHVG）. The results indicated that the sediment samples of five different layers contained only five groups of archaea. Achaeal community diversity was low, and dominant archaea had some differences in each layers.