山绿茶总黄酮抑制实验性大鼠良性前列腺增生的作用观察

Inhibition of Hainan holly leaf extract on rats with experimental benign prostatic hyperplasia

48只雄性SD大鼠随机分为正常对照组、模型组、前列康组和山绿茶总黄酮高、中、低剂量组(80,40,20 mg/kg)6组,对去势大鼠注射丙酸睾酮造成的良性前列腺增生症(BPH)模型(模型组去势注射丙酸睾酮,对照组去势未注射丙酸睾酮),用前列康和山绿茶总黄酮高、中、低剂量对前列腺增生模型大鼠灌胃;模型组、对照组以生理盐水灌胃,连续5周。测定大鼠前列腺湿重比,大鼠血清总酸性磷酸酶(ACP)、非前列腺特异性酸性磷酸酶(NPAP),计算前列腺特异性酸性磷酸酶(PAP)。光学显微镜下观察前列腺组织的形态变化。结果表明,山绿茶总黄酮高、中剂量组(80,40 mg/kg)的前列腺湿重指数、血清PAP活性与模型组比较均明显降低,差异有显著性(P<0.01),与前列康组相似;显微镜下山绿茶总黄酮高剂量(80 mg/kg)对上皮细胞形态与模型组相比有明显改变:腺腔中分泌物积聚,可见前列腺结石,与正常组的前列腺细胞结构基本相接近。说明山绿茶总黄酮具有抑制前列腺增生的作用。

48 rats were randomly divided into 6 groups：a normal control group,a model group of benign prostatic hyperplasia（BPH） treated by subcutaneous injection of testosterone propionate,a positive control group（rat in model group treated with Qianliekang by orally） and Hainan holy leaf extract（HHLE） groups（rat in model group treated with HHLE by orally）.After treating 5 weeks,the rat prostate wet weight,the index of prostate gland（PI）,the serum acid phosphatase（ACP）,non-prostate-specific acid phosphatase（NPAP） and PAP were measured.Morphological changes of the prostate tissue under optical microscope were observed.Results showed that prostate wet weight,PI and serum PAP of high and medium dose groups were obviously decreased compared with the model group,with a significant difference（P0.01） similar to that of Qianliekang.High-doses group（80 mg/kg） improved the morphological changes of the prostate characterized by reducing interstitial fibrosis,increasing secretions in the glandular cavity and prostate calculi showing eosinophilic,which were similar to those of the normal group.HHLE has inhibitive effect on BPH induced by testosterone propionate in rats.