心脑康对抗脾切除术后大鼠血小板升高的临床观察

Observation of Curative Effects of Xinnaokang Capsule on Rat's Thrombocytosis after Splenectomy

目的观察中药心脑康对脾切除术后大鼠血小板升高的抑制效果。方法选择90只大鼠制作Wistar大鼠脾切除模型,随机分为治疗组、对照组、空白组3组,每组30例。治疗组术后予中药心脑康25 mg/kg,每日1剂;对照组术后予阿司匹林5.0 mg/kg,每日1次喂服;空白组术后予0.9%氯化钠注射液2 ml,每日1次喂服。术后10、30、90 d监测3组血白细胞数、血小板计数、凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)并对比分析。结果治疗组、对照组、空白组外周血白细胞不同时间点均显著升高,组间比较差异无统计学意义(P>0.05)。治疗组、对照组血小板在10、30、90 d均显著降低,组内比较差异均有统计学意义(P<0.05),同一时间点两组间比较差异有统计学意义(P<0.05);与空白对照组同一时间点比较差异亦有统计学意义(P<0.05)。治疗组、对照组PT、APTT、TT均较空白组延长,与空白组比较差异有统计学意义(P<0.05)。治疗组与对照组在不同时间点比较差异均有统计学意义(P<0.05)。治疗组总有效率为96.7%,对照组为86.7%,差异有统计学意义(P<0.05)。结论应用中药心脑康治疗脾切除术后血小板升高效果优于阿司匹林,可明显降低血栓的发病率。

Objective To study the inhibition effects of Xinnaokang Capsule on rat＇s thrombocytosis after splenectomy in order to reduce the complications due to coagulation defects after surgery. Methods 90 rats were selected to establish splenectomy models of Wistar rats. The rats were divided into treatment group, control group and the blank group （30 in each）. In the treatment group, Xinnaokang Capsule（25 mg/kg）was given once a day. In the control group, Aspirin（5.0 mg/kg） was given once a day. In the blank group, physiological saline（2 ml） was given once a day. The WBC, PLT, PT, APTTand Tr were recorded on 10, 30, 90 d after operation, and the difference of the 3 groups were compared. Results WBC increased obviously in different times, and there was no difference among the 3 groups （P 〉 0. 05）. PLT was reduced obviously in the treatment group and the control group （P 〈 0.05）, and the treatment group was significantly lower than the control group （P 〈 0. 05）, the treatment group and the control group were significantly lower than the blank group at the same time （P 〈0. 05）. The PT, APTT and TT of the treatment group and control group were significantly longer than that of the blank group （P 〈 0.05）. There was significant difference between the treatment group and the control group on the 10th, 30th and 90th day （P 〈0. 05）. The total effective rate of the treatment group was 96.7%, and 86.7% in the control group （P 〈0. 05）. Conclusion The application of Xinnaokang Capsule in the treatment of thrombocytosis after splenectomy can make the PLT return to normal and obviously reduce the incidence rate of the thrombus. The effect is better than that of Aspirin.