苹果渣制备饲料酵母和嗜热链球菌

Study on co-culture of Yeast and Streptococcus.thermophilus with Apple Pomace

以苹果渣为主料,分别单独和混合培养饲料酵母菌和嗜热链球菌。探讨了各自在不同温度下的生长曲线,并在此基础上研究了混合培养的可行性。结果显示:单独发酵活菌总数可达4.32×108 cfu/ml,分段恒温发酵(35℃下培养,先接入4%酵母菌培养2d,再接入2%嗜热链球菌)活菌总数可达6.53×108 cfu/ml,分段变温培养(32℃下先接入4%酵母菌培养2d,再将温度调制37℃接入2%嗜热链球菌)活菌总数可达7.21×108 cfu/ml,没有较大差异。由此可见,利用苹果渣混合培养酵母菌和嗜热链球菌确实可行。

With apple pomace-based materials, separate and mixed culture of yeast and Streptococcus thermophilus （S. T） was studied. The respective growth curves of them under different temperature were discussed, and on this basis, the feasibility of the mixed culture was studied. The results indicated that ： in separate fermentation the total number of viable cells was up to 4.32×10^8 cfu/ml, in staged constant-temperature-fermentation （first 4% yeast inoculated and cultured at 35＂C for 2 d, then 2% S. T inoculated） the total number of viable was up to 6.53×10^8 cfu/ml, and in staged variable-temperature-fermentation（first 4% yeast inoculated and cultured at 32℃ for 2 d, then up to 37℃, 2% of S. T inoculated） the total number of viable cells was up to 7.21 ; 10s cfu/ml, there were no major differences. Thus, the use of apple pomace mixed culture of yeasts and S. T was indeed feasible.