杂交玉米农大108及其亲本种子内生细菌群落的多样性

Diversity of Endophytic Bacterial Communities in Seeds of Hybrid Maize(Zea mays L.,Nongda108) and Their Parental Lines

【目的】研究不同基因型玉米对其种子内生细菌群落结构的影响。【方法】通过构建16S rDNA文库的非培养方法,对杂交玉米组合农大108正、反交及其亲本种子内生细菌群落多样性进行研究。【结果】两后代种子内生细菌分别含46和42个OTU,第一优势菌属均为Pseudomonas,丰度分别为34.29%和34.00%;亲本玉米黄C和178种子内生细菌分别含17和11个OTU,第一优势菌属分别为Enterobacter及Burkholderia,丰度分别为49.13%和89.72%。母本玉米黄C种子内生细菌群落中Roseateles、Enterobacter、Pseudomonas及Sphingomonas4个菌属与父本178种子内生细菌群落中Pseudomonas、Ralstonia、Paenibacillus及Bacillus 4个菌属存在其正交后代种子中。母本178种子内生细菌群落中Acinetobacter、Pseudomonas、Ralstonia、Paenibacillus及Bacillus 5个菌属与父本黄C种子内生细菌群落中Roseateles、Citrobacter、Pantoea、Pseudomonas、Stenotrophomonas及Escherichia 6个菌属存在其反交后代种子中。【结论】遗传相关的杂交玉米农大108正、反交后代其亲本在内生细菌群落结构具有一定的关联性。

【Objective】 The objective of this study is to investigate the influence of genotypes of maize on seeds endophytic bacterial communities.【Method】 Using the 16S rDNA clone library technique,this paper conducted a priliminary study on the community diversity of endophytic bacteria in seeds of reciprocal cross hybrid maize（Zea mays L.,Nongda108） and their parental lines.【Result】 The endophytic bacterial communities in 108A and 108B included 46 and 42 OTUs respectively,and the abundance of the first dominant bacterium Pseudomonas in 108A and 108B was 34.29% and 34.00%.The parental lines Huang C and 178 included 17 and 11 OTUs respectively,the abundance of Enterobacter and Burkholderia as the first dominant bacterium in which respectively was 49.13% and 89.72%.Roseateles,Enterobacter,Pseudomonas and Sphingomonas found in the seeds of 108A were also existed in its female parent Huang C.Pseudomonas,Ralstonia,Paenibacillus and Bacillus were also existed in its male parent 178.Acinetobacter,Pseudomonas,Ralstonia,Paenibacillus and Bacillus found in the seeds of 108B were also existed in its female parent 178.Roseateles,Citrobacter,Pantoea,Pseudomonas,Stenotrophomonas and Escherichia were also existed in its male parent Huang C.【Conclusion】 In the seeds of reciprocal cross hybrid maize,Nongda 108 and their parental lines which are genetically related,the endophytic bacterial community structure has certain relevance.