摘要
【目的】研究鼠李糖乳酸杆菌(Lactobacillus rhamnosus)对氧化应激状态下Caco-2细胞抗氧化功能的影响。【方法】将培养的Caco-2细胞分为4组:对照组和氧化应激组(在培养液中加入100μmol.L-1 H2O2),处理组Ⅰ和处理组Ⅱ在氧化应激条件下分别添加鼠李糖乳酸杆菌(约108 CFU.mL-1)和抗氧化剂特丁基对苯二酚(TBHQ)(2.75μg.mL-1)各1 mL。Caco-2细胞培养至12和48 h时分别测定其上清液和裂解液的抗氧化活性。【结果】添加鼠李糖乳酸杆菌减轻了Caco-2细胞氧化受损,使细胞培养上清中的总抗氧化力(T-AOC)显著高于氧化应激组:12 h时显著提高了细胞培养上清谷胱甘肽过氧化物酶(GSH-Px)(P<0.01)、过氧化氢酶(CAT)(P<0.01)活力以及细胞裂解液中超氧化物酶(SOD)活力(P<0.01)和还原型谷胱甘肽(GSH)含量(P<0.05),48 h时显著提高了细胞培养上清液抗O2-(ASAFR)(P<0.01)、GSH-Px(P<0.01)、CAT(P<0.01)、SOD活力(P<0.01)和细胞裂解液中过氧化物酶(POD)活力(P<0.01),丙二醛(MDA)含量显著降低(P<0.01)。添加TBHQ组12 h时细胞上清中T-AOC(P<0.01)和CAT活性(P<0.01)及细胞裂解液中GSH含量(P<0.01)显著高于氧化应激组,而处理48 h后相应指标低于氧化应激组;此时,细胞培养上清液中MDA含量极显著降低(P<0.01),抗O2-、SOD、GSH-Px、POD活力和细胞裂解液中POD活力显著升高(P<0.05)。【结论】在体外条件下,鼠李糖乳酸杆菌可以提高氧化应激状态下Caco-2细胞的抗氧化功能。
【Objective】This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress.【Method】 Caco-2 cells were randomly divided into 4 groups,control group and oxidative stress group(100 μmol?L-1 H2O2),treatment groupsⅠandⅡ were added with 1 mL Lactobacillus rhamnosus(about 108 CFU?mL-1) and tertiary butylhydroquinone(TBHQ)(2.75 μg?mL-1) under oxidative stress,respectively.Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured.【Result】 Compared to oxidative stress group,the total antioxidation capacity(T-AOC),the glutathione peroxidase(GSH-Px)(P0.01),catalase(CAT)(P0.01) activities in the cultured supernatants,and the superoxide dismutase(SOD) activity(P0.01),and the glutathione(GSH) content(P0.05) in the cells lysates at 12 h in treatment groupⅠincreased,and the increases of the antisuperoxide anion radical(O2-)(P0.01),GSH-Px(P0.01),CAT(P0.01),SOD(P0.01) activities in the cells culture supernatants and the peroxidase(POD) activity(P0.01) and the decreases of malondialdehyde(MDA) content(P0.01) in the cells lysates at 48 h were also observed.In treatment groupⅡ,the T-AOC(P0.01),CAT activity(P0.01) in the cells culture supernatants and the glutathione(GSH) content(P0.01) in the cells lysates at 12 h increased significantly,but these values and the MDA content at 48 h decreased significantly(P0.01).The anti-superoxide anion radical(O2-),SOD,GSH-Px,POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased(P0.05).【Conclusion】These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第23期4926-4932,共7页
Scientia Agricultura Sinica
基金
国家重点基础研究发展计划(2009CB118705)
浙江省重大科技专项(2006C12086)