鼠李糖乳酸杆菌对Caco-2细胞抗氧化功能的影响

Antioxidative Function of Lacbacillus rhamnosus to Caco-2 Cells

【目的】研究鼠李糖乳酸杆菌(Lactobacillus rhamnosus)对氧化应激状态下Caco-2细胞抗氧化功能的影响。【方法】将培养的Caco-2细胞分为4组:对照组和氧化应激组(在培养液中加入100μmol.L-1 H2O2),处理组Ⅰ和处理组Ⅱ在氧化应激条件下分别添加鼠李糖乳酸杆菌(约108 CFU.mL-1)和抗氧化剂特丁基对苯二酚(TBHQ)(2.75μg.mL-1)各1 mL。Caco-2细胞培养至12和48 h时分别测定其上清液和裂解液的抗氧化活性。【结果】添加鼠李糖乳酸杆菌减轻了Caco-2细胞氧化受损,使细胞培养上清中的总抗氧化力(T-AOC)显著高于氧化应激组:12 h时显著提高了细胞培养上清谷胱甘肽过氧化物酶(GSH-Px)(P<0.01)、过氧化氢酶(CAT)(P<0.01)活力以及细胞裂解液中超氧化物酶(SOD)活力(P<0.01)和还原型谷胱甘肽(GSH)含量(P<0.05),48 h时显著提高了细胞培养上清液抗O2-(ASAFR)(P<0.01)、GSH-Px(P<0.01)、CAT(P<0.01)、SOD活力(P<0.01)和细胞裂解液中过氧化物酶(POD)活力(P<0.01),丙二醛(MDA)含量显著降低(P<0.01)。添加TBHQ组12 h时细胞上清中T-AOC(P<0.01)和CAT活性(P<0.01)及细胞裂解液中GSH含量(P<0.01)显著高于氧化应激组,而处理48 h后相应指标低于氧化应激组;此时,细胞培养上清液中MDA含量极显著降低(P<0.01),抗O2-、SOD、GSH-Px、POD活力和细胞裂解液中POD活力显著升高(P<0.05)。【结论】在体外条件下,鼠李糖乳酸杆菌可以提高氧化应激状态下Caco-2细胞的抗氧化功能。

【Objective】This experiment was conducted to study the antioxidative function of Lactobacillus rhamnosus to Caco-2 cells under oxidative stress.【Method】 Caco-2 cells were randomly divided into 4 groups,control group and oxidative stress group（100 μmol？L-1 H2O2）,treatment groupsⅠandⅡ were added with 1 mL Lactobacillus rhamnosus（about 108 CFU？mL-1） and tertiary butylhydroquinone（TBHQ）（2.75 μg？mL-1） under oxidative stress,respectively.Antioxidative activities in culture supernatants and lysates of Caco-2 cells at 12 h and 48 h were measured.【Result】 Compared to oxidative stress group,the total antioxidation capacity（T-AOC）,the glutathione peroxidase（GSH-Px）（P0.01）,catalase（CAT）（P0.01） activities in the cultured supernatants,and the superoxide dismutase（SOD） activity（P0.01）,and the glutathione（GSH） content（P0.05） in the cells lysates at 12 h in treatment groupⅠincreased,and the increases of the antisuperoxide anion radical（O2-）（P0.01）,GSH-Px（P0.01）,CAT（P0.01）,SOD（P0.01） activities in the cells culture supernatants and the peroxidase（POD） activity（P0.01） and the decreases of malondialdehyde（MDA） content（P0.01） in the cells lysates at 48 h were also observed.In treatment groupⅡ,the T-AOC（P0.01）,CAT activity（P0.01） in the cells culture supernatants and the glutathione（GSH） content（P0.01） in the cells lysates at 12 h increased significantly,but these values and the MDA content at 48 h decreased significantly（P0.01）.The anti-superoxide anion radical（O2-）,SOD,GSH-Px,POD activities in the cells culture supernatants and the POD activity in the cells lysates at 48 h increased（P0.05）.【Conclusion】These results indicated that adding Lactobacillus rhamnosus could increase the antioxidative function of Caco-2 cells under oxidative stress under in vitro conditions.