运用多座位外显方差法分析中国荷斯坦牛CXCR1和IL8基因与乳房炎易感性的关系

Using the MPVA Model to Analyze the Impact of Gene-Gene Interaction between CXCR1 and IL8 2789/2862 on the Risk of Mastitis Susceptibility of Chinese Holstein

旨在运用多座位外显方差分析法(MPVA)分析中国荷斯坦牛CXCR1和IL8两个基因多个突变位点多态性与乳房炎易感性的交互作用。本研究以南方某大型奶牛场634头(其中102头患乳房炎)中国荷斯坦牛为试验材料,采用PCR-SSCP和直接测序法分析CXCR1-5′端和编码区及IL8 2789/2862内含子3、外显子4和5,2个基因共7个SNPs位点的遗传多态性,并用MPVA法分析CXCR1和IL8基因各位点突变及其基因型组合对奶牛乳房炎易感性的影响。结果,MPVA分析发现CXCR1-1830、CXCR1-1768和IL8 2789/2862三位点交互作用对奶牛乳房炎易感性的影响达到显著水平。CXCR1-1830、CXCR1-1768和IL8 2789/2862三位点交互模型作为奶牛乳房炎易感性相关的基因联合选择模型最佳,MPVA可用于奶牛乳房炎易感性多基因分析模型。

The aims of this study were to identify the interaction of CXCR1 and IL8 and their impacts on the risk of mastitis susceptibility of Chinese Holstein,and to test the feasibility of using Multi-Locus penetrance variance analysis（MPVA） model in analyzing gene-gene interactions.The polymorphisms of 7 SNP sites including the 5′ flanking region and coding region of CXCR1 and intron 3,exon 4 and exon 5 of IL8 were detected by PCR-SSCP and direct sequencing for 634 Chinese Holstein,including 102 individual with mastitic,from a northern farm of China,and to identify the interaction of CXCR1 and IL8 and their impacts on the risk of mastitis susceptibility using MPVA.The interactions of CXCR1-1830-1768-IL8 2789/2862 showed significant effects on the mastitis susceptibility of cow using MPVA model.The results indicate that the interactions of CXCR1-1830-1768-IL8 2789/2862 is the best multi-genotype model to estimate the risk of mastitis susceptibility of Chinese Holstein.MPVA can be used to estimate the risk of mastitis susceptibility of Chinese Holstein.