蛇足石杉化学成分研究

Chemical constituents of Huperzia serrata

目的研究蛇足石杉Huperzia serrata的化学成分。方法利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱及半制备高效液相色谱等方法分离纯化;通过核磁共振谱、质谱等光谱数据鉴定化合物结构。结果分离得到10个化合物,分别鉴定为β-谷甾醇(1)、5,7,4′-三羟基-3′-甲氧基黄酮(2)、5,7,4′-三羟基-3′,5′-二甲氧基黄酮(3)、芹菜素(4)、正三十烷醇(5)、21β-hydroxy-serrat-14-en-3β-ol(6)、21α-hydroxy-serrat-14-en-3β-ol(7)、16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate(8)、3β,21α-dihydroxy-serrat-14-en-24-ol(9)、3α,21β-dihydroxy-serrat-14-en-24-ol(10)。结论化合物2～5为首次从本植物中分离得到。

Objective To investigate the chemical constituents of Huperzia serrata.Methods The constituents were isolated by silica gel column,Sephadex LH-20 gel column,medium pressure column,and semi-preparative HPLC chromatographies,and their structures were elucidated by chemical properties and spectroscopic analyses.Results The structures of ten compounds were isolated and identified as β-sitosterol（1）,5,7,4′-trihydroxy-3′-methoxyflavone（2）,5,7,4′-Trihydroxy-3′,5′-dimethoxyflavone（3）,apigenin（4）,n-triacontanol（5）,21β-hydroxy-serrat-14-en-3β-ol（6）,21α-hydroxy-serrat-14-en-3β-ol（7）,16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate（8）,3β,21α-dihydroxy-serrat-14-en-24-ol（9）,and 3α,21β-dihydroxy-serrat-14-en-24-ol（10）.Conclusion Compounds 2—5 are obtained from this plant for the first time.