摘要
目的研究蛇足石杉Huperzia serrata的化学成分。方法利用硅胶柱色谱法、Sephadex LH-20凝胶柱色谱法、中压柱色谱及半制备高效液相色谱等方法分离纯化;通过核磁共振谱、质谱等光谱数据鉴定化合物结构。结果分离得到10个化合物,分别鉴定为β-谷甾醇(1)、5,7,4′-三羟基-3′-甲氧基黄酮(2)、5,7,4′-三羟基-3′,5′-二甲氧基黄酮(3)、芹菜素(4)、正三十烷醇(5)、21β-hydroxy-serrat-14-en-3β-ol(6)、21α-hydroxy-serrat-14-en-3β-ol(7)、16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate(8)、3β,21α-dihydroxy-serrat-14-en-24-ol(9)、3α,21β-dihydroxy-serrat-14-en-24-ol(10)。结论化合物2~5为首次从本植物中分离得到。
Objective To investigate the chemical constituents of Huperzia serrata.Methods The constituents were isolated by silica gel column,Sephadex LH-20 gel column,medium pressure column,and semi-preparative HPLC chromatographies,and their structures were elucidated by chemical properties and spectroscopic analyses.Results The structures of ten compounds were isolated and identified as β-sitosterol(1),5,7,4′-trihydroxy-3′-methoxyflavone(2),5,7,4′-Trihydroxy-3′,5′-dimethoxyflavone(3),apigenin(4),n-triacontanol(5),21β-hydroxy-serrat-14-en-3β-ol(6),21α-hydroxy-serrat-14-en-3β-ol(7),16-oxo-21β-hydroxy-serrat-14-en-3α-yl-acetate(8),3β,21α-dihydroxy-serrat-14-en-24-ol(9),and 3α,21β-dihydroxy-serrat-14-en-24-ol(10).Conclusion Compounds 2—5 are obtained from this plant for the first time.
出处
《中草药》
CAS
CSCD
北大核心
2011年第12期2407-2409,共3页
Chinese Traditional and Herbal Drugs