均匀设计法优选白花刺参的提取工艺研究

Optimization of extracting condition for Morina nepalensis by uniform design

目的优选白花刺参的最佳回流提取工艺条件。方法采用UV法和HPLC法测定总皂苷及刺参苷J的量,以总皂苷提取率、刺参苷J提取率和干浸膏收率为综合评价指标,采用U8(84)均匀设计表设计试验,考察乙醇体积分数、乙醇用量、提取次数、提取时间4种因素对总皂苷提取的影响。结果白花刺参的最佳提取工艺参数:80%乙醇回流提取2次,每次1 h。结论均匀设计法优选出的白花刺参总皂苷的提取工艺合理可行,为白花刺参总皂苷的进一步研究提供参考。

Objective To optimize the extractive condition of Morina nepalensis by hot refluxing.Methods HPLC and UV were established to determine the monepaloside J and total saponins.U8（84） Uniform design form was selected as the experiments’ schedule,the total saponins,monepaloside J and the yield was used as evaluation indicator to and the ethanol concentration,amount of extraction solvent added,extractive time and times of extraction was used as the 4 influencing factors.Results The optimum condition was the extraction in 80% ethanol,two times and 1 h.Conclusion The optimal extracting method of the total saponins of M.nepalensis is suitable and feasible by uniform design.The study could provide scientific references for further investigation of M.nepalensis.