硫酸化人参皂苷体外抗新城疫病毒作用

Antiviral activity of sulfated ginsenoside on Newcastle disease virus in vitro

目的观察硫酸化人参皂苷(sGS)抗新城疫病毒的活性。方法采用氯磺酸-吡啶法制备sGS,红外光谱仪对sGS结构进行分析,MTT法检测硫酸化修饰前后人参皂苷(GS)预先给药、接种病毒后给药、与病毒同时给药3种不同给药方式的抗新城疫病毒活性。结果硫酸化修饰后人参皂苷在1 144、807 cm?1有2个特征吸收峰,表明硫酸基已经和糖链结合成酯。预先给药或病毒接种后给药,加入一定质量浓度范围内的GS和sGS的CEF培养液的A570值均显著大于未给药的病毒组(P<0.05),且GS与sGS的抗病毒活性无显著差异(P>0.05);GS和sGS与病毒同时给药,两药在一定质量浓度范围内的A570值也均显著大于未给药的病毒组(P<0.05),且sGS的最大病毒抑制率显著高于GS。结论 GS经硫酸化修饰后可提高其抗新城疫病毒活性,主要表现为提高其直接杀灭病毒的作用。

Objective In order to observe the antiviral activity of sulfated ginsenoside（sGS） on Newcastle disease virus（NDV）.Methods sGS was prepared by cholorsulfonic acid-pyridine method and the structure of sGS was analyzed by FTIR spectra.Then,the antiviral activity of ginsenosides（GS） and sGS was determined with MTT by three modes.Results The results showed that two characteristic absorptive peak presented at 1 144 and 807 cm？1,respectively,which proved that the sulfated group had linked with polysaccharide to form sulfate ester.In pre-adding drug and post-adding drug modes： the A570 values of GS and sGS in CEF culture medium were significantly higher than those of virus control at some concentration（P0.05）,but it was not significant difference between GS and sGS（P0.05）.In simultaneous adding drug and NDV,the A570 values of GS and sGS were significantly higher than those of virus control at some concentration（P0.05） and the maximum virus inhibitory rate of sGS was significantly higher than that of GS.Conclusion The antiviral activity of GS on NDV could be improved with sulfation,which appears to improve the effect on directly killing the virus.