续断提取物对非酒精性脂肪肝小鼠调脂保肝作用及其有效部位研究

Hypolipidemic function with liver protection of extracts from Dipsacus asper on nonalcoholic fatty liver disease in mice and its activie fractions

目的研究续断提取物对喂饲高脂饲料所致非酒精性脂肪肝小鼠的调脂保肝作用,并初步确定其有效部位。方法给小鼠喂饲高脂饲料建立非酒精性脂肪肝小鼠模型。观察续断水提物、70%乙醇提取物(粗提物B)及其不同提取部位(B-1、B-2、B-3)对非酒精性脂肪肝小鼠血清肝功能指标、血脂及肝组织病理学等的影响。结果与模型组比较,给予续断粗提物B和B-2后,小鼠血清天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、丙二醛(MDA)、游离脂肪酸(FFA)水平以及体脂和肝脏指数均显著降低(P<0.05、0.01),SOD水平显著升高(P<0.01);血清总胆固醇(TC)、低密度脂蛋白(LDL)显著下降(P<0.01),高密度脂蛋白(HDL)显著升高(P<0.01);肝细胞脂肪变性显著修复。结论续断提取物对高脂饲料所致非酒精性脂肪肝小鼠具有保肝调脂作用,其有效部位B-2能显著降低非酒精性脂肪肝小鼠肝脏内脂肪聚积和脂质过氧化损伤,明显修复脂肪肝病变。

Objective To investigate the hypolipidemic function with liver protection of the extract from the roots of Dipsacus asper and its active fractions on treating high fat diet（HFD）-induced nonalcoholic fatty liver disease（NAFLD） in mice.Methods A model of NAFLD,HFD fed Kunming mice,was established to investigate the effects of seventy percent of ethanol crude extract B and its different fractions B-1,B-2,and B-3 on the indexes of liver function,lipids,and liver histopathology in serum of mice with NAFLD.Results The model of HFD-induced NAFLD in mice have successfully produced.The AST,ALT,MDA,and FFA in serum of mice administered the extracts B and B-2 from the roots of D.asper were significantly decreased（P0.05 and 0.01）,besides lipids and liver indexes.The SOD activity was higher than that of mice in model group（P0.01）,TC and LDL in serum were reduced（P0.01）,and HDLwas increased（P0.01） obviously.And then,the pathobiology of liver dramatically showed the repairing of hepatic steatosis.Conclusion The extracts from the roots of D.asper has the hypolipideic function with liver protection on HFD-induced NAFLD in mice,especially fraction B-2 could reduce hepatic lipogenesis and lipid peroxidation reaction,and to recover the fatty liver diseases.