骨肉瘤组织中Ⅰ、Ⅱ、和Ⅲ型胶原蛋白的表达

Expression of type Ⅰ，Ⅱ and Ⅲ collagen in human osteosarcoma

目的：研究人骨肉瘤组织中Ⅰ、Ⅱ和Ⅲ型胶原的表达及其与骨肉瘤分型和分化的关系，并对胶原的两种染色方法进行比较。方法：用饱和苦ｋ味酸．天狼星红－偏振光镜和免疫组化LSAN法，检测46例人骨肉瘤组织中Ⅰ、Ⅱ和Ⅲ型胶原的表达情况，并用EMAL－200真彩图像分析系统进行分析和半定量测定。结果：骨肉瘤组织中不仅有Ⅰ型胶原的表达（阳性率87％，灰度值171．99±14．74），且出现了Ⅱ型和Ⅲ型胶原（阳性率分别为30．4％和43．4％，灰度值分别为153．071±18．82和168．29±18．36）；与对照的骨瘤组织比较，骨肉瘤Ⅰ型胶原表达明显减弱；Ⅱ型胶原在软骨母细胞型和混合型的表达明显高于其它型骨肉瘤（P＜0．of），而Ⅰ、Ⅲ型胶原的表达在各型骨肉瘤间差异无显著性；在高中低3组不同分化程度的骨肉瘤间，Ⅰ型胶原表达差异有显著性（P＜001），分化越低表达越少；天狼星红与免疫组化染色结果基本一致。结论：Ⅱ型胶原可作为软骨母细胞型和混合型骨肉瘤的分型参考指标，Ⅰ型胶原的表达可作为骨肉瘤分化及恶性度判断的参考指标。天狼星红染色是鉴别Ⅰ、Ⅲ型胶原简便、经济的方法之一。

Purpose To investigate expression of type Ⅰ、Ⅱand Ⅲ collagen in human osteosarcoma andtheir relationship with classification and differentiation of osteosarcoma, at the same time to compare two meth-ods of staining collagen. Methods Type Ⅰ、Ⅱ and Ⅲ collagen in 46 cases of osteosarcoma were detected usingpicric-siriusred-polariscope and immunohistochemistry(IHC); EMAL-200 image software was used to analyzestaining result. Results Expression of type Ⅰ collagen in osteosarcoma (positive rate 87 %, grey value 171. 99± 14. 74) was significantly weaker than that in control osteoma. Besides type Ⅰ, there were also expression oftype Ⅱ and Ⅲ collagen in osteosarcoma (positive rate are respectively 30. 43 % and 43. 5 %, grey value respec-tively 153. 07 ± 18. 82 and 168. 29 ± 18. 36); Type Ⅱ collagen stain was significantly stronger in chondroblasticand mixed osteosarcomas than other types. Among three groups of osteosarcomas of high, moderate and low de-gree, the difference of type Ⅰ collagen was statistically significant, the lower osteosarcoma differentiated, theweaker expression of type Ⅰ collagen was. The results of both Sirius-Red and IHC stain were same. ConclusionType Ⅱ collagen can be utilized as one of indicators to identify chondroblastic and mixed osteosarcomas; TypeⅠ collagen can be used as one of markers to determine the differentiation of osteosarcoma; Picro-Sirius-red-po-lariscope is a simple and ecnomic method to identify type Ⅰ and type Ⅲ collagen.