5种禽类卵黄低密度脂蛋白对猪精子冷冻效果的影响

Comparative Study of LDL Extracted from Five Avian Species on the Cryopreservation of Boar Sperm

为了确定具有最佳抗冷冻效果的禽类卵黄低密度脂蛋白(LDL)及其添加质量分数,在猪精液冷冻稀释液中分别添加质量分数为6%、7%、8%、9%和10%的鸡、鸭、鹌鹑、鸽子和鸵鸟的卵黄LDL,分析不同禽类的LDL对猪精子的冷冻保存效果。结果表明,稀释液中添加质量分数为9%的鸡、鸭、鹌鹑、鸽子卵黄LDL以及质量分数8%的鸵鸟卵黄LDL时,冷冻-解冻后精子活率最高,分别达到42.33%、35.63%、31.47%、47.33%和36.40%。以5种禽类LDL最佳质量分数配制冷冻稀释液冷冻精子,发现质量分数为9%的鸽蛋LDL冻存猪精子时解冻后精子活率达到47.33%,顶体完整性达到62.57%,质膜完整性达到48.13%,均显著优于其他处理组(P<0.05)。说明鸽子卵黄LDL对猪精子具有良好的冷冻保护性能,可提高猪精子抵抗低温打击的能力。

Low density lipolipid（LDL） can protect sperm from freezing damage during the sperm frozen-thawed process.In order to distinguish the efficiency of LDL extracted from different avian species,to confirm which one and how much of it has the best anti-freezing effect,LDL extracted from eggs of hen,duck,quail,pigeon and ostrich with the mass fraction of 6%,7%,8%,9% and 10% was added into the cryopreservation diluents of boar sperm separately.The result showed that after the frozen-thawed process,LDL extracted from eggs of hen,duck,quail and pigeon with concentration of 9% and ostrich LDL with concentration of 8% could protect sperm with highest livability of 42.33%,35.63%,31.47%,47.33% and 36.40%,respectively.They were significantly better than that of other concentrations（P〈0.05）.Among the cryopreservation diluents and cryopreservation sperm prepared with the five avian species with the optimum concentration,it was found that the 9% pigeon LDL group showed 47.33% sperm livability,62.57% acrosome integrity and 48.13% membrane integrity after thawing,all of them were better than that of other groups（P〈0.05）.Based on the most significant increase of the cold resistance ability that conferred by pigeon egg yolk LDL,it has a good performance on sperm cryopreservation.