芡实多糖的分离纯化及抗氧化作用研究

Study on methods of purifying polysaccharides in semen eurylie and its antioxidant effect

目的研究芡实多糖的分离纯化方法及其抗氧化作用。方法用AB-8大孔树脂纯化芡实粗多糖,用Fenton法和邻苯三酚自氧化法测定芡实多糖的体外抗氧化作用。结果芡实粗多糖经大孔树脂纯化后多糖含量为83.82%;芡实多糖对羟自由基(OH.)和超氧阴离子(O2-.)均有一定的清除作用,且其作用强度随芡实多糖浓度增大而增加。结论 AB-8大孔吸附树脂可以用来纯化芡实多糖,芡实多糖具有一定的抗氧化作用,本实验结果为芡实多糖的纯化及进一步开发提供了依据。

Objective To study the methods of purifying polysaccharides in Semen Eurylie and its antioxidant effect. Methods The polysaccharides were purified by AB- 8 macroporous resin, And its antioxidant effect was determined with Fenton method and autoxidation of pyrogallol method. Results The content of polysaccharides was 83.82 % after purified by macroporous resin. The purified polysaccharides had some effect on eliminating hydroxyl free radical and superoxide anion and their antioxidant capacity was dose--dependent with concentration. Conclusion AB-8 resin can be used to purify polysaccharides in Semen Eurylie and the purified polysaccharides had a certain antioxidant effect. Practically, the experimental results provide a basis for further development of Semen Eurylie.