牛AR基因5’UTR SNP分析及与精液品质关系

SNP Analysis of 5＇-UTR of Androgen Receptor Gene and the Relationship with Semen Quality in Bulls

通过PCR—SSCP方法对种公牛群体雄激素受体（AR）基因5’非翻译区（5’UTR）多态性进行检测，结果表明：AR基因5＇UTR含有多态性位点；测序结果显示5＇UTR-1575处存在T—G碱基突变；用最小二乘分析方法对该基因型和生产性状相关分析显示遗传多态性与精子密度性状呈显著相关，AA型显著高于AB型（P=0．025），对其他性状的影响差异不显著。本实验为研究种公牛体内AR的生物学作用奠定了基础，为肉用种公牛的早期选种选育提供了候选基因和分子标记。

The polymorphism of AR gene 5＇UTR in bulls was detected by PCR-SSCP. The sequencing resuits indicated that there was one single nucleotide mutation （T to G） in the 5＇UTR -1575 of the AR gene; The genetic correlations were between genotypes and performances was obtained by the least square analysis, the resuits showed that genetic polymorphisms significantly correlated with sperm count performance. And AA genotype was higher than AB genotype （P=0.025）. Other traits were not significantly affected. The research laid the foundation for the future studies of the biological function of AR gene in bulls and theoretical basis for breeding of meat bulls.