铁缺乏对大鼠血脂和肝脏脂质过氧化的影响

Effect of Iron Deficiency on Serum Lipids and Lipid Peroxidation of Liver

目的探讨铁缺乏对血脂水平和肝脏脂质过氧化的影响。方法选择健康SPF/VAF级初断乳SD雄性大鼠30只,按体重随机分为两组:对照组(饲料铁含量93.3mg/kg)和铁缺乏组(饲料铁含量9.6mg/kg),每组15只。通过控制大鼠铁摄入量,制备缺铁动物模型。喂养6周后,测量体质量,股动脉放血处死动物,分离血清,摘取肝脏称重,并计算肝脏相对质量。采用生化法检测血清三酰甘油(TG)、总胆固醇(TC)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、血红蛋白、血清铁水平和总铁结合力;制备肝脏匀浆,检测谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果与对照组相比,铁缺乏组大鼠血红蛋白降低(t=30.919,P<0.01),达到中重度贫血水平;体质量、肝脏绝对质量和相对质量均降低(t=4.393,9.068,8.805,P均<0.01);血清TC、HDL和LDL水平均降低(t=9.017,9.519,3.880,P均<0.01);MDA含量较对照组减低(t=2.810,P<0.05)。结论铁缺乏可能通过干扰肝细胞的正常脂代谢与抗氧化系统,对大鼠体内血脂及脂质过氧化产生影响。

Objective To study the effect of iron deficiency on serum lipids and lipid peroxidation of liver by controlling iron intake of rats. Methods Male SD rats were randomly divided into two groups： the control group （the iron content of feeds was 93.3 mg/kg） and iron deficiency group （the iron content was 9.6 mg/kg）, 15 in each groups. After 6 weeks treatment, the rats were killed by exsanguinations. The blood was sampled to separate serum and the livers were harvested to measure absolute liver mass and relative liver mass. The blood samples were analyzed for triglyceride （TG）, total cholesterol （TC）, high density lipoprotein （HDL）, low density lipoprotein （LDL）, hemoglobin, serum iron levels and total iron binding capacity by biochemical method. Then livers were prepared to homogenate, and tested for GPx, SOD and MDA. Results Compared with the control group, the level of serum hemoglobin in iron deficiency group was decreased （t=30.919, P〈0.01） and it reached the level of moderate to severe anemia. The levels of body mass, absolute liver mass and relative liver mass were lower （t=4.393, 9.068, 8.805, respectively, P〈0.01）; the levels of serum TC, HDL and LDL were lower （t=9.017, 9.519, 3.880, respectively, P〈0.01）; the level of SOD was decreased, and the level of MDA was lower than the control group （t=2.810, P〈0.05）. Conclusion Iron deficiency may effect on serum lipids and lipid peroxidation of liver in rats by interfering with the fat metabolism and antioxidant system of normal liver cells.