姜黄素对博莱霉素致小鼠急性肺损伤的影响

Effect of Curcumin on Bleomycin-induced Acute Lung Injury in Mice

目的探讨姜黄素对博莱霉素(BLM)诱导的小鼠急性肺损伤(ALI)的治疗作用。方法昆明雄性小鼠60只,分为假手术组、模型组、姜黄素高、中、低剂量组,每组12只。除假手术组外其余各组小鼠气管内1次性滴注盐酸博莱霉素,假手术组1次性滴注等体积的生理盐水。造模后24h开始给药,持续到处死动物的前1天。姜黄素高、中、低剂量组给药剂量分别为200、100、50mg/(kg·d),假手术组、模型组分别灌服等体积的生理盐水。各组动物分别于给药后第3天和第7天各随机处死6只,观察肺组织形态学改变,测定谷胱甘肽过氧化物酶(GSH-Px)、诱导型一氧化氮合酶(iNOS)含量。结果姜黄素能降低肺组织中iNOS含量,提高小鼠体内GSH-Px含量。与假手术组相比,第3、7天时模型组iNOS含量明显增高(P<0.01),GSH-Px含量明显降低(P<0.01)。姜黄素中、高剂量组和模型组相比,iNOS含量显著下降(P<0.01),GSH-Px含量显著增加(P<0.01)。病理组织学检查亦表明,一定剂量的姜黄素可明显减轻肺组织损伤的程度。结论 100～200mg/(kg·d)姜黄素在BLM诱导的小鼠急性肺损伤中有一定的防治作用。其机制可能与通过升高肺组织的GSH-Px水平,降低iNOS的水平,重建机体氧化、抗氧化平衡有关。

Objective To investigate the curative effect of curcumin on acute pulmonary injury （ALI） induced by bleomycin （BLM） in mice. Methods 60 Kunming male mice were divided into sham operation group, model group, curcumin high, medium and low dose group, and 12 in each group. Except the sham group mice given 1-time intratracheal infusion of equal volume of saline, all other group mice were given 1-time intratracheal infusion of bleomycin. From the second day of the model set up to the day the animals were sacrificed, curcumin high-, medium-, low-dose group were administered with curcumin at doses of 200, 100, 50 mg/（kg· d）, and the sham operation group and model group were fed with an equal volume of saline. Half of mice of all groups were respectively killed at the 3rd and 7th day, while their lung tissues were incised to make pathological sections which were stained with HE, and contents of GSH-Px and iNOS were measured. Data was shown as mean ＋ standard deviation （x±s） and analyzed with SPSSll.0 statistical software. Results Curcumin was able to increase the content of GSH-Px and reduce the activity of iNOS; Compared with the sham group, contents of iNOS at the 3rd and 7th day, was significantly higher （P〈O.01） and GSH-Px decreased significantly （P〈0.O1）; But compared with the model group, the contents of iNOS in the medium- and high-dose curcumin group decreased and GSH-Px increased significantly （all P〈O.01）. The lung pathological tests also showed obvious improvement in mice with curcumin treatment. Conclusion 100-200 mg/（kg·d） curcumin has protective effect at the early phase of ALI. The mechanism may be related with the increase of GSH-Px and the decrease of iNOS in lung tissue, and with the reconstruction of oxidative and anti-oxidative balance.