浙江首例人粒细胞无形体病患者病原学分析

Pathogenic analysis of the first case of hmnan granulocytic anaplasmosis in Zhejiang Province

目的对浙江首例疑似人粒细胞无形体病（HI强）患者进行病原学诊断与分子生物学分析。方法运用分子生物学方法检测吞噬细胞无形体16SrRNA，间接免疫荧光法（IrA）检测患者临床症状期和恢复健康3个月后血清吞噬细胞无形体IgG抗体。结果患者发热期血液PCR获得的16SrRNA基因片段：282bp和389bp，符合目标片段大小。后者经与GenBank已登录的国内外嗜吞噬细胞无形体株对比，同源性为99．73％。患者血清临床症状期吞噬细胞无形体IgG抗体阳性，效价为1：128，恢复健康3个月后抗体滴度呈4倍下降。结论浙江省首次病原学证实1例人粒细胞无形体病患者，其感染株与已知嗜吞噬细胞无形体株16SrRNA无明显差异。

Objective To take etiological diagnosis and molecular biological analysis for the first case of human granulocytic anaplasmosis in Zhejiang Province. Methods A. phagocytophilum 16S rRNA was detected and sequenced by molecular biological metyods. Serum A. phagocytophilum IgG antibody in clinical stage and 3 months after recovery were detected by indirect immonufluorescence technique. Results The PCR products of the expected size, 282 bp and 389 bp were obtain in blood from the patient at pyrogenic stage. The 389 bp nucleotide sequence was compared with A. phagocytophilum 16S rRNA nucleotides previously published in GenBank. The homology was 99.73%. Serum IgG antibody to A. phagocytophilum was positive in 1 ： 128 titer at pyrogenic stage and had 4-fold decline after convalescence. The first case of human grarnulocytic anaplasmosis is confirmed in Zhejiang Province. There is no obvious differece between 16S rRNA from the patient and A. phagocytophilum known before.