山核桃外果皮黄酮提取与纯化

Extraction and Purification of Flavone in C. cathayensis Exocarp

采用有机溶剂萃取法提取山核桃外果皮黄酮化合物,通过单因素和正交试验确定了最佳工艺参数。结果表明,提取溶剂为体积分数70%乙醇、提取时间4 h、提取温度60℃、液料比15 mL/g条件下得黄酮化合物最高提取率为1.14%。采用硅胶色谱柱层析法纯化黄酮化合物,在液固比3 mL/g的条件下,体积分数70%乙醇以2 mL/min的流速进行洗脱,黄酮纯度提高了34.6%,总黄酮回收率达89.46%。

In order to study the extraction and purification of flavonein C.cathayensis exocarp,organic solvent extraction was used to extrat flavone compound in C.cathayensis exocarp.Through single fraction experiments and orthogonal experiments,the optimal technological parameters were determined.The result showed the maximum extraction ration of flavone compound was 1.14% under the condition of 70% ethanol,time of 4 h,temperature of 60℃,liquid-solid ratio of 15 mL/g.Silica gel chromatography was used to purify falconoid,liquid-solid ratio of 3 mL/g,gradient eluted by 70% ethanol at the speed of 2 mL/min,the purity of flavone was improved by 34.6%,and its recovery reached to 89.46%.