摘要
采用有机溶剂萃取法提取山核桃外果皮黄酮化合物,通过单因素和正交试验确定了最佳工艺参数。结果表明,提取溶剂为体积分数70%乙醇、提取时间4 h、提取温度60℃、液料比15 mL/g条件下得黄酮化合物最高提取率为1.14%。采用硅胶色谱柱层析法纯化黄酮化合物,在液固比3 mL/g的条件下,体积分数70%乙醇以2 mL/min的流速进行洗脱,黄酮纯度提高了34.6%,总黄酮回收率达89.46%。
In order to study the extraction and purification of flavonein C.cathayensis exocarp,organic solvent extraction was used to extrat flavone compound in C.cathayensis exocarp.Through single fraction experiments and orthogonal experiments,the optimal technological parameters were determined.The result showed the maximum extraction ration of flavone compound was 1.14% under the condition of 70% ethanol,time of 4 h,temperature of 60℃,liquid-solid ratio of 15 mL/g.Silica gel chromatography was used to purify falconoid,liquid-solid ratio of 3 mL/g,gradient eluted by 70% ethanol at the speed of 2 mL/min,the purity of flavone was improved by 34.6%,and its recovery reached to 89.46%.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2011年第12期177-181,共5页
Transactions of the Chinese Society for Agricultural Machinery
基金
安徽省高校省级自然科学基金重点资助项目(KJ2010A293
2006KJ190B)
关键词
山核桃外果皮
黄酮
提取
纯化
C.cathayensis exocarp
Flavone
Extraction
Purification