环介导等温扩增反应的原理及应用

Principle and Application of Loop-mediated Isothermal Amplification

环介导等温扩增反应(Loop-mediated isothermal amplification,LAMP)是日本学者Notomi于2000年在NucleicAcids Res杂志上公开的一种新的基因诊断技术,该法操作简便,扩增效率及灵敏度高,已广泛应用于食品、临床、农业等领域,有望成为主流的基因诊断方法。本文对LAMP的反应特征及原理、优缺点、临床应用作一综述。

Loop-mediated isothermal amplification（LAMP） is a novel technique for genetic diagnosis,which was published on Nucleic Acids Res in 2000 by Notomi,a Japanese scientist.It is a simple,efficient and sensitive DNA amplification method which has been widely used in various fields including food,clinic and agriculture,and might be a mainstream method for genetic diagnosis.This paper reviews the reaction characters,principle,advantage,disadvantage as well as clinical application of LAMP.