摘要
目的应用甲状腺细针吸取细胞学进行取样,通过液基细胞学方法对标本进行处理,结合免疫组化方法,探讨对甲状腺乳头状癌诊断有价值的诊断指标。方法对85名甲状腺乳头状癌患者进行FNA取样后,应用液基薄层细胞学方法,分析一组免疫标记物CK-19、Galectin-3、HBME1、CD-44v、CD-56和E-Cad-herin与甲状腺乳头状癌的关系。结果 CK-19、Galectin-3、HBME1、CD-44v表达阳性分别为78例(91.8%)、74例(87.1%)、64例(75.3%)和73例(85.9%),CD-56和E-cadherin的失表达分别为81例(95.3%)和80例(94.1%)。结论液基细胞学检查能提高甲状腺乳头状癌诊断的准确性;CK-19、Galectin-3、HBME-1和CD-44v的阳性表达对甲状腺乳头状癌有诊断意义,为诊断甲状腺乳头状癌提供了一种可靠而有前景的诊断方法。
[ Objective ] To explore the diagnostic value of papillary carcinoma indicators by the methods of thyroid fine-needle aspiration cytology, liquid-based cytology, and immunohistoehemical methods. [ Methods ] A number of 85 fine needle aspirations, positive for papillary thyroid cancer were studied using Thin Layer Cytology. A panel of the immune markers CK-19, Galectin-3, HBME1, CD-44v, CD-56, and E-eadhefin was performed. [Resuits] Positive expression of CK-19 was observed in 78 (91.8%), of Galeetin-3 in 74 cases (87.1%), HBME1 in 64 cases (75.3%), and of CD-44v in 73 cases (85.9%). Loss of expression of CD-56 was observed in 81 cases (95.3%) and of E-cadherin in 80 cases (94.1%). [ Conclusions ] The diagnostic accuracy of thyroid papillary carcinoma can be improved by liquid based cytology can improve. CK-19, Galectin-3, HBME-1 and CD-44v positive expression were significant for the diagnosis of papillary thyroid cancer, moreover they provides a reliable and promising diagnostic method for it.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2011年第27期3380-3382,3386,共4页
China Journal of Modern Medicine