11461例妇科门诊患者HPV亚型感染状况的研究

Research of HPV infection situation among 11461 cases of gynecological clinic patients

目的了解妇科门诊患者HPV感染率、亚型分布以及不同年龄HPV感染状况,为防治HPV感染提供科学依据。方法对2008年10月～2011年7月来该院妇科门诊就诊的11 461例患者进行登记,并采集其阴道、宫颈分泌物作为标本,用导流杂交基因芯片技术进行21种HPV基因型的分型检测。用EXCEl 2007和SPSS 17.0软件建立数据库及统计分析,HPV感染率比较采用统计学χ2检验。结果①HPV阳性总检出率为44.45%(5 094/11 461),高危型别感染率为40.54%(4 646/11 461),低危型别感染率为4.26%(488/11 461),单一型别感染率为30.89%(3 540/11 461),多重型别感染率为14.00%(1 604/11 461);②HPV感染率最高的亚型为HPV16型(15.55%),且以单一感染和双重感染为主;③不同年龄检查者的HPV感染率差异有统计学意义(χ2=77.03,P<0.01),随着年龄的增加,HPV的感染率逐渐增加(趋势性χ2=76.67,P<0.01),不同年龄的HPV感染者感染不同的高低亚型构成上有统计学意义(χ2=121.70,P<0.01),不同年龄的HPV感染者单一及多重感染构成上也具有统计学意义(χ2=121.70,P<0.01),各年龄HPV感染者均以单一及高危感染为主,但在30岁以下及60岁以上年龄HPV感染者中高低危合并多重感染高于其他年龄段。结论该院妇科门诊患者HPV感染率处于较高水平,HPV感染率最高的亚型为HPV16。不同年龄检查者HPV感染率不同,且随年龄增加有逐渐增加趋势,高低危亚型、单一及多重感染的构成在不同的年龄中有差别,30岁以下及60岁以上年龄女性中,高低危合并的多重感染高于30～60岁的女性。

[ Objective ] To learn the HPV infection rate, subtype distribution and HPV infection status in different age among gynecological elinie patients, so as to provide a scientific basis for the prevention and control of HPV infection. [Methods] Cervical tissue speeimens from 11 461 gynecological clinic patients were examined with Flowthrough Hybridization and gene chip technique （cape medical system） to determine the 21 HPV genotypes. Making use of the Excel 2007 and SPSS 17.0 software to establish database and analyze data, the Chi-square test was used in the comparison of the HPV infeetion rate between different age groups. [Results] ① Of the 11 461 specimens, HPV was detected in 5 096 specimens with a positive rate of 44.45% （5096/11461）, the high-risk HPV infection rate was 40.54% （4646/11461）, the low-risk HPV infection was 4.26% （488/11461）, single subtype infection rate was30.89% （3540/11461）, and complex subtypes infection rate was 14.00% （1604/11461）. ② Of all the subtypes in this study, HPV16 was highest infection rate of subtype （15.55%）, and with the single and double infection primarily. ③ The difference was significant in the HPV infection rate between different ages （X2=77.03, P 〈0.01）. Along with the increasing age, HPV infection rates increase gradually （X2=76.67, P 〈0.01）, the constituent ratio of the HPV infections from different age group was significant different on the distribution of the high-risk and low-risk HPV subtypes （X2=121.70, P 〈0.01）, and the HPV single and complex infection （X2=121.70, P 〈0.01）.the single and high-risk HPV infection was the main infection in different age groups. In the age group of less than 30 years and more than 60 years, the constituent ratio of the high-risk and low-risk co-infection is higher than other groups. [ Conclusion ] HPV infection rates in our hospital gynecological clinic was at high level, the highest subtype in the HPV infection rate was the HPV16. The HPV infection varied in different age groups, and increased with the age increasing. The distribution of high-risk and low-risk HPV infection as well as the single and complex infection was different between various ages. In the age group of less than 30 years and more than 60 years, the constituent ratio of the highrisk and low-risk co-infection is higher than 30-60 years old infection.